Obesity is a metabolic imbalance characterized by excessive deposition of white fat. The browning of white fat can effectively treat obesity and related diseases. Although Dlgap1 (Discs, Large (Drosophila) Homolog-Associated Protein 1) is suspected to have an effect on this process, no empirical evidence is available. To understand the role of Dlgap1, we cultured white and brown fat cells, then performed overexpression and knockout experiments. We found that Dlgap1 overexpression in brown adipocytes inhibits brown-fat-related gene expression, promotes white-fat-related genes, while also increasing brown-adipocyte proliferation and apoptosis. However, the gene overexpression has no effect on brown adipocyte maturation. Knocking out Dlgap1 in white fat cells promotes the expression and inhibition of brown-fat-related and white-fat-related genes, respectively. Additionally, the knockout inhibits white fat cell proliferation and apoptosis, while also promoting their maturation. Dlgap1 negatively regulates the browning of white adipocytes by influencing cell proliferation and apoptosis.

中文翻译：

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Dlgap1通过影响细胞增殖和凋亡来负调控白脂肪细胞的褐变

肥胖是一种代谢失衡，其特征在于白色脂肪的过多沉积。白色脂肪褐变可以有效治疗肥胖症和相关疾病。尽管怀疑Dlgap1（Discs，大（果蝇）同源相关蛋白1）对该过程有影响，但没有经验证据。为了了解Dlgap1的作用，我们培养了白色和棕色的脂肪细胞，然后进行了过表达和敲除实验。我们发现棕色脂肪细胞中的Dlgap1过表达抑制了棕色脂肪相关基因的表达，促进了白色脂肪相关基因的表达，同时还增加了棕色脂肪细胞的增殖和凋亡。但是，基因过表达对棕色脂肪细胞的成熟没有影响。敲除白色脂肪细胞中的Dlgap1可促进棕发相关基因和白发相关基因的表达和抑制，分别。另外，基因敲除抑制了白色脂肪细胞的增殖和凋亡，同时还促进了它们的成熟。Dlgap1通过影响细胞增殖和凋亡来负调控白脂肪细胞的褐变。