Areas with saline soils are sparsely populated and have fragile ecosystems, which severely restricts the sustainable development of local economies. Zoysia grasses are recognized as excellent warm-season turfgrasses worldwide, with high salt tolerance and superior growth in saline-alkali soils. However, the mechanism underlying the salt tolerance of Zoysia species remains unknown. The phenotypic and physiological responses of two contrasting materials, Zoysia japonica Steud. Z004 (salt sensitive) and Z011 (salt tolerant) in response to salt stress were studied. The results show that Z011 was more salt tolerant than was Z004, with the former presenting greater K+/Na+ ratios in both its leaves and roots. To study the molecular mechanisms underlying salt tolerance further, we compared the transcriptomes of the two materials at different time points (0 h, 1 h, 24 h, and 72 h) and from different tissues (leaves and roots) under salt treatment. The 24-h time point and the roots might make significant contributions to the salt tolerance. Moreover, GO and KEGG analyses of different comparisons revealed that the key DEGs participating in the salt-stress response belonged to the hormone pathway, various TF families and the DUF family. Zoysia salt treatment transcriptome shows the 24-h and roots may make significant contributions to the salt tolerance. The auxin signal transduction family, ABA signal transduction family, WRKY TF family and bHLH TF family may be the most important families in Zoysia salt-stress regulation.

中文翻译：

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生理和转录组分析揭示了结缕草耐盐的机制

盐碱地地区地广人稀，生态系统脆弱，严重制约当地经济的可持续发展。结缕草是世界公认的优良暖季型草坪草，具有高耐盐性，在盐碱土壤中生长优良。然而，结缕草物种耐盐性的机制仍不清楚。两种对比材料结缕草的表型和生理反应。研究了 Z004（盐敏感）和 Z011（耐盐）对盐胁迫的响应。结果表明，Z011 比 Z004 更耐盐，前者的叶和根中 K+/Na+ 比率更高。为了进一步研究耐盐性的分子机制，我们比较了盐处理下两种材料在不同时间点（0小时、1小时、24小时和72小时）和不同组织（叶和根）的转录组。24小时时间点和根可能对耐盐性有显着贡献。此外，不同比较的GO和KEGG分析表明，参与盐胁迫反应的关键DEG属于激素途径、各种TF家族和DUF家族。结缕草盐处理转录组显示24小时和根可能对耐盐性做出重大贡献。生长素信号转导家族、ABA信号转导家族、WRKY TF家族和bHLH TF家族可能是结缕草盐胁迫调节中最重要的家族。