• Perennials have a complex shoot architecture with axillary meristems organized in zones of differential bud activity and fate. This includes zones of buds maintained dormant for multiple seasons and used as reservoirs for potential growth in case of damage. The shoot of Arabis alpina , a perennial relative of Arabidopsis thaliana , consists of a zone of dormant buds placed between subapical vegetative and basal flowering branches. This shoot architecture is shaped after exposure to prolonged cold, required for flowering.
• To understand how vernalization ensures the maintenance of dormant buds, we performed physiological and transcriptome studies, followed the spatiotemporal changes of auxin, and generated transgenic plants.
• Our results demonstrate that the complex shoot architecture in A. alpina is shaped by its flowering behavior, specifically the initiation of inflorescences during cold treatment and rapid flowering after subsequent exposure to growth‐promoting conditions. Dormant buds are already formed before cold treatment. However, dormancy in these buds is enhanced during, and stably maintained after, vernalization by a BRC1‐dependent mechanism. Post‐vernalization, stable maintenance of dormant buds is correlated with increased auxin response, transport, and endogenous indole‐3‐acetic acid levels in the stem.
• Here, we provide a functional link between flowering and the maintenance of dormant buds in perennials.

中文翻译：

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春化塑造了芽的结构，并确保了多年生阿拉比斯高山植物中休眠芽的维持

• 多年生植物具有复杂的芽结构，在芽活动和命运不同的区域组织了腋生分生组织。其中包括芽保持多个季节处于休眠状态的区域，并在受损时用作潜在生长的贮藏库。的拍摄高山南芥，多年生相对拟南芥，由放置近尖端无性和基底花枝之间休眠芽的区。暴露于开花所需要的长时间寒冷后，这种芽的结构便会成形。
• 为了了解春化处理如何确保休眠芽的维持，我们进行了生理和转录组研究，跟随生长素的时空变化，并生成了转基因植物。
• 我们的结果表明，高山被孢霉的复杂芽构型受其开花行为影响，特别是冷处理期间的花序启动和随后暴露于促进生长的条件后的快速开花。休眠芽在冷处理之前已经形成。但是，通过BRC1依赖性机制，在春化过程中可以增强这些芽的休眠，并在其进行稳定化之后保持这些休眠。春化后，休眠芽的稳定维持与茎中生长素应答，转运和内源性吲哚-3-乙酸水平增加有关。
• 在这里，我们提供多年生植物开花与维持休眠芽之间的功能性联系。