Transient receptor potential melastatins (TRPMs) are most well known as cold and menthol sensors, but are in fact broadly critical for life, from ion homeostasis to reproduction. Yet, the evolutionary relationship between TRPM channels remains largely unresolved, particularly with respect to the placement of several highly divergent members. To characterize the evolution of TRPM and like channels, we performed a large-scale phylogenetic analysis of >1,300 TRPM-like sequences from 14 phyla (Annelida, Arthropoda, Brachiopoda, Chordata, Cnidaria, Echinodermata, Hemichordata, Mollusca, Nematoda, Nemertea, Phoronida, Priapulida, Tardigrada, and Xenacoelomorpha), including sequences from a variety of recently sequenced genomes that fill what would otherwise be substantial taxonomic gaps. These findings suggest: 1) the previously recognized TRPM family is in fact two distinct families, including canonical TRPM channels and an eighth major previously undescribed family of animal TRP channel, TRP soromelastatin; 2) two TRPM clades predate the last bilaterian–cnidarian ancestor; and 3) the vertebrate–centric trend of categorizing TRPM channels as 1–8 is inappropriate for most phyla, including other chordates.

中文翻译：

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系统发育鉴定了两个真后生动物 TRPM 进化枝和第八个 TRP 家族，TRP 索美司他汀 (TRPS)。


瞬态受体电位褪黑素 (TRPM) 最广为人知的是冷感和薄荷醇传感器，但实际上对生命（从离子稳态到生殖）广泛至关重要。然而，TRPM 渠道之间的演化关系在很大程度上仍未得到解决，特别是在几个高度分歧的成员的安置方面。为了表征 TRPM 和类似通道的进化，我们对来自 14 个门（环节动物门、节肢动物门、腕足动物门、脊索动物门、刺胞动物门、棘皮动物门、半索动物门、软体动物门、线虫门、纽虫门、磷门动物）的 >1,300 个 TRPM 样序列进行了大规模系统发育分析。 、 Priapulida、Tardigrada 和 Xenacoelomorpha），包括来自各种最近测序的基因组的序列，这些序列填补了原本巨大的分类学空白。这些发现表明：1) 先前公认的 TRPM 家族实际上是两个不同的家族，包括经典 TRPM 通道和第八个先前未描述的动物 TRP 通道主要家族，TRP 索美司他汀 (TRP soromelastatin)； 2）两个TRPM进化枝早于最后的两侧对称动物-刺胞动物祖先； 3) 以脊椎动物为中心的将 TRPM 通道分类为 1-8 的趋势对于大多数门（包括其他脊索动物）来说是不合适的。