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Microbial community and interspecies interaction during grazing of ark shell bivalve (Scapharca subcrenata) in a full-scale bioremediation system of mariculture effluents.
Marine Environmental Research ( IF 3.3 ) Pub Date : 2020-03-12 , DOI: 10.1016/j.marenvres.2020.104956
Betina Lukwambe 1 , Regan Nicholaus 2 , Li Zhao 3 , Wen Yang 3 , Jinyong Zhu 3 , Zhongming Zheng 3
Affiliation  

A novel biological approach using ark shell bivalves as potential species for remediation of effluents was studied to determine the microbial community interspecies interaction and nutrient cycling in a restoration system of mariculture effluents. A field study showed that Scapharca subcrenata was the main driver of the microbial community's interspecies-interaction (PERMANOVA, R = 0.0572, P = 0.005) in the treatment zone (TZ). Analysis of co-occurrence networks based on random matrix theory (RMT) indicated that the network's complexity parameters were enhanced in the TZ and disrupted in the control zone (CZ) due to eutrophic disturbances. Concurrently, the TZ was correlated with more profound network modifications (i.e., higher modularity, total nodes (n), cohesion, and proportion of positive links), suggesting that S. subcrenata influenced microbial interspecies interactions in the system. Similarly, the co-occurring networks of generalists Proteobacteria (OTU2037) at genus Anaerospora and Actinobacteria (OTU9660) at genus Candidatus aquiluna for anaerobic ammonia-oxidation (ANAMMOX) were highly significant in the TZ. The top-down and bottom-up forces of S. subcrenata influenced the removal efficiency of nitrogenous compounds by reducing 81.51% of nitrite (NO2--N), 84.61% of total ammonium nitrogen (TAN) and 72.78% of nitrate (NO3--N). Generally, the introduction of ark shell bivalve (S. subcrenata) to the system as a biofilter provides a very low-cost bioremediation technology that could be one of the best restorations and remediation tools for mariculture effluents.

中文翻译:

在海水养殖废水的大规模生物修复系统中,放牧方舟壳双壳类动物(Scapharca subcrenata)期间的微生物群落和种间相互作用。

研究了一种使用方舟壳双壳类生物作为污水修复的潜在物种的新型生物学方法,以确定海水养殖废水恢复系统中微生物群落的种间相互作用和养分循环。现场研究表明,Scapharca subcrenata是处理区(TZ)中微生物群落种间相互作用的主要驱动力(PERMANOVA,R = 0.0572,P = 0.005)。基于随机矩阵理论(RMT)的共现网络分析表明,网络的复杂性参数在TZ中得到增强,而在控制区(CZ)中由于富营养化干扰而被破坏。同时,TZ与更深刻的网络修改(即更高的模块性,总节点数(n),内聚性和正向链接的比例)相关,这表明S。蛛网膜下层影响了系统中微生物间的相互作用。同样,在厌氧氨氧化(ANAMMOX)中,厌氧菌属的变形杆菌(OTU2037)和产假丝酵母菌(Candidatus aquiluna)的放线菌(OTU9660)共同存在的网络在TZ中非常重要。葡萄球菌的自上而下和自下而上的作用力通过减少81.51%的亚硝酸盐(NO2--N),84.61%的总铵氮(TAN)和72.78%的硝酸盐(NO3- -N)。通常,将方舟壳双壳类生物(S. subcrenata)作为生物滤池引入该系统提供了一种非常低成本的生物修复技术,该技术可能是海水养殖废水的最佳修复和修复工具之一。在TZ中,厌氧氨氧化菌属(Anaerospora)属的变形杆菌(OTU2037)和产假丝酵母(Candidatus aquiluna)属放线菌(OTU9660)的共生网络在TZ中非常重要。葡萄球菌的自上而下和自下而上的作用力通过减少81.51%的亚硝酸盐(NO2--N),84.61%的总铵氮(TAN)和72.78%的硝酸盐(NO3- -N)。通常,将方舟壳双壳类生物(S. subcrenata)作为生物滤池引入该系统提供了一种非常低成本的生物修复技术,该技术可能是海水养殖废水的最佳修复和修复工具之一。在TZ中,厌氧氨氧化菌属(Anaerospora)属的变形杆菌(OTU2037)和产假丝酵母(Candidatus aquiluna)属放线菌(OTU9660)的共生网络在TZ中具有很高的意义。葡萄球菌的自上而下和自下而上的作用力通过减少81.51%的亚硝酸盐(NO2--N),84.61%的总铵氮(TAN)和72.78%的硝酸盐(NO3- -N)。通常,将方舟壳双壳类生物(S. subcrenata)作为生物滤池引入该系统提供了一种非常低成本的生物修复技术,该技术可能是海水养殖废水的最佳修复和修复工具之一。
更新日期:2020-03-12
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