The adverse effects of triphenyltin (TPT) on aquatic systems have attracted much attention because TPT is widely used and prevalent in aquatic environments. Here, zebrafish embryos/larvae were exposed to TPT (0, 0.039, 0.39, and 3.9 nM; 0, 15, 150 and 1500 ng/L) for 7 or 14 days to determine its toxic effects on the hypothalamic–pituitary–thyroid (HPT) axis. The results showed that whole-body total T4 and T3 levels were significantly decreased, which was accompanied by the significant upregulation of the expression of the dio1, dio2 and ugt1ab genes after exposure to TPT for 7 and 14 days. Genes related to thyroid hormone synthesis (crh, tshβ, nis, tpo and tg) were upregulated at both 7 and 14 days after TPT exposure. This might have been due to the positive feedback regulation of the HPT axis, which is caused by a decrease in thyroid hormone in the whole body in zebrafish. In addition, the survival rates and body lengths were reduced after treatment with TPT for 7 and 14 days. This indicated that TPT caused adverse effect on the development of zebrafish embryos/larvae. In summary, the results suggested that TPT caused thyroid disruption and developmental toxicity in zebrafish larvae.

三苯基锡（TPT）对水生系统的不利影响已引起人们的广泛关注，因为TPT在水生环境中得到了广泛使用和普遍使用。在这里，斑马鱼胚胎/幼虫暴露于TPT（0、0.039、0.39和3.9 nM； 0、15、150和1500 ng / L）持续7或14天，以确定其对下丘脑-垂体-甲状腺（ HPT）轴。结果表明，暴露于TPT 7天和14天后，全身总T4和T3水平显着降低，同时伴随着dio1，dio2和ugt1ab基因表达的显着上调。与甲状腺激素合成相关的基因（crh，tshβ，nis，tpo和tgTPT暴露后第7天和第14天均被上调）。这可能是由于HPT轴的正反馈调节所致，这是由于斑马鱼体内整个体内的甲状腺激素减少所致。此外，TPT治疗7天和14天后，存活率和体长降低。这表明TPT对斑马鱼胚胎/幼虫的发育造成了不利影响。总之，该结果表明TPT导致斑马鱼幼虫的甲状腺破坏和发育毒性。