Polydopamine/carboxylic graphene oxide-composited polypyrrole films for promoting adhesion and alignment of Schwann cells.,Colloids and Surfaces B: Biointerfaces

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Polydopamine/carboxylic graphene oxide-composited polypyrrole films for promoting adhesion and alignment of Schwann cells.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-03-12 , DOI: 10.1016/j.colsurfb.2020.110972
Ya Li ₁ , Zhongbing Huang ₁ , Ximing Pu ₁ , Xianchun Chen ₁ , Guangfu Yin ₁ , Yulin Wang ₁ , Deqiang Miao ₁ , Junbing Fan ₂ , Jiachen Mu ₁

Affiliation

Polydopamine (PDA)- and carboxylic graphene oxide (CGO)-composited polypyrrole (PPy) films were prepared via electrospinning of poly-l-lactic acid (PLLA), electrodeposition of PPy blended with CGO and polymerization of dopamine (PDA/CGO/PPy-PLLA), and its surface conductivity and elastic modulus reached to ∼17.3 S/cm and ∼260 MPa, respectively. PDA composition could maintain the higher conduction and elastic modulus of this film after the immersion of 3 w, due to the stronger force between PDA molecule and CGO sheets or PPy particles. The results of cell experiments indicated the better adhesion and higher expression of neural proteins in rat Schwann cells (RSCs) on PDA/CGO/PPy-PLLA films, because the composition of PDA provided more absorption and immobilization site for proteins and the regular coating of PDA particles on the CGO sheets reduced the potential damage of Graphene derivatives on the cell membrane integrity. Electrical stimulation (ES) could facilitate ∼31 % of RSCs to align along the current direction on PDA/CGO/PPy-PLLA films, significantly higher than those on PPy-PLLA and CGO/PPy-PLLA films. A mechanism about the cell alignment on films with ES was proposed: the movement of the cytomembrane proteins under ES and their linkage with serum proteins immobilized by PDA facilitated the extension of growth cone along the ES direction.

中文翻译：

聚多巴胺/氧化石墨烯复合的聚吡咯膜，用于促进雪旺细胞的粘附和排列。

通过电纺丝聚乳酸（PLLA），PPy与CGO混合电沉积以及多巴胺（PDA / CGO / PPy）聚合制备聚多巴胺（PDA）和氧化石墨烯（CGO）复合的聚吡咯（PPy）薄膜-PLLA），其表面电导率和弹性模量分别达到〜17.3 S / cm和〜260 MPa。由于PDA分子与CGO片材或PPy颗粒之间的作用力较大，因此PDA组合物在浸泡3 w后可以保持该膜的较高的导电性和弹性模量。细胞实验结果表明，在PDA / CGO / PPy-PLLA膜上，大鼠雪旺细胞（RSC）中神经蛋白具有更好的粘附力和更高的表达，因为PDA的成分为蛋白质提供了更多的吸收和固定位点，并且PDA颗粒在CGO板上的规则涂层减少了石墨烯衍生物对细胞膜完整性的潜在损害。电刺激（ES）可以促进约31％的RSC在PDA / CGO / PPy-PLLA膜上沿电流方向排列，远高于PPy-PLLA和CGO / PPy-PLLA膜上的RSC。提出了一种在ES膜上进行细胞排列的机制：ES下细胞膜蛋白的移动以及它们与PDA固定的血清蛋白的连接促进了生长锥沿ES方向的延伸。电刺激（ES）可以促进约31％的RSC在PDA / CGO / PPy-PLLA膜上沿电流方向排列，远高于PPy-PLLA和CGO / PPy-PLLA膜上的RSC。提出了一种在ES膜上进行细胞排列的机制：ES下细胞膜蛋白的移动以及它们与PDA固定的血清蛋白的连接促进了生长锥沿ES方向的延伸。电刺激（ES）可以促进约31％的RSC在PDA / CGO / PPy-PLLA膜上沿电流方向排列，远高于PPy-PLLA和CGO / PPy-PLLA膜上的RSC。提出了一种在ES膜上进行细胞排列的机制：ES下细胞膜蛋白的移动以及它们与PDA固定的血清蛋白的连接促进了生长锥沿ES方向的延伸。

更新日期：2020-03-12

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