Enhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques,Crystal Growth & Design

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Enhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques
Crystal Growth & Design ( IF 3.2 ) Pub Date : 2020-03-12 , DOI: 10.1021/acs.cgd.9b00969
M. Polino ₁ , C. A. M. Portugal ₁ , H. Le The ₂ , R. Tiggelaar ₃ , J. Eijkel ₂ , J. G. Crespo ₁ , I. M. Coelhoso ₁ , M. P. Pina _{4,

5} , R. Mallada _{4,

5}

Affiliation

In this work, the influence of surface topography on protein crystallization over Nafion is investigated. Two types of Nafion-based membranes were modified by soft lithographic techniques in order to create different topographies at the micro and nano scale and subsequently tested. From an analysis of the induction time, nucleation, and crystal growth rate of trypsin from bovine pancreas, all of the patterned Nafion-based membranes show an enhanced nucleation and crystal growth. To provide additional insight into the experimental observations, the wettability properties of the prepared samples and the ratio of the Gibbs free energy of heterogeneous nucleation to homogeneous nucleation were evaluated. The crystallization outcome results from the combined effect of both the structural and chemical properties of the nucleant Nafion surface.

中文翻译：

低成本表面构图技术修饰的Nafion膜上增强的蛋白质结晶

在这项工作中，研究了表面形貌对Nafion上蛋白质结晶的影响。通过软光刻技术对两种基于Nafion的膜进行了改性，以便在微米和纳米尺度上产生不同的形貌，并随后进行了测试。从牛胰胰蛋白酶的诱导时间，成核和晶体生长速率分析，所有基于Nafion的图案化膜均显示出增强的成核和晶体生长。为了提供对实验观察结果的进一步了解，评估了制备样品的润湿性和异质成核与均相成核的吉布斯自由能之比。结晶结果是由nucleant Nafion表面的结构和化学性质共同作用所致。

更新日期：2020-04-23

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