Effect of hemolysis on Fourier transform infrared and Raman spectra of blood plasma.,Journal of Biophotonics

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Effect of hemolysis on Fourier transform infrared and Raman spectra of blood plasma.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-04-07 , DOI: 10.1002/jbio.201960173
Dinesh K R Medipally _{1,

2} , Daniel Cullen _{1,

2} , Valérie Untereiner _{3,

4} , Jane Bryant ₁ , Ganesh D Sockalingum ₃ , Thi N Q Nguyen _{1,

2} , Emma Noone ₅ , Shirley Bradshaw ₅ , Marie Finn ₅ , Mary Dunne ₅ , Aoife M Shannon ₆ , John Armstrong _{6,

7} , Aidan D Meade _{1,

2} , Fiona M Lyng _{1,

2}

Affiliation

Hemolysis is a very common phenomenon and is referred as the release of intracellular components from red blood cells to the extracellular fluid. Hemolyzed samples are often rejected in clinics due to the interference of hemoglobin and intracellular components in laboratory measurements. Plasma and serum based vibrational spectroscopy studies are extensively applied to generate spectral biomarkers for various diseases. However, no studies have reported the effect of hemolysis in blood based vibrational spectroscopy studies. This study was undertaken to evaluate the effect of hemolysis on infrared and Raman spectra of blood plasma. In this study, prostate cancer plasma samples (n = 30) were divided into three groups (nonhemolyzed, mildly hemolyzed, and moderately hemolyzed) based on the degree of hemolysis and FTIR and Raman spectra were recorded using high throughput (HT)‐FTIR and HT‐Raman spectroscopy. Discrimination was observed between the infrared and Raman spectra of nonhemolyzed and hemolyzed plasma samples using principal component analysis. A classical least square fitting analysis showed differences in the weighting of pure components in nonhemolyzed and hemolyzed plasma samples. Therefore, it is worth to consider the changes in spectral features due to hemolysis when comparing the results within and between experiments.

中文翻译：

溶血对血浆的傅立叶变换红外光谱和拉曼光谱的影响。

溶血是一种非常普遍的现象，称为细胞内组分从红细胞释放到细胞外液中。由于实验室测量中血红蛋白和细胞内成分的干扰，经常在临床上拒绝溶血样品。基于血浆和血清的振动光谱研究被广泛应用于生成各种疾病的光谱生物标志物。但是，尚无研究报道溶血在基于血液的振动光谱研究中的作用。进行这项研究以评估溶血对血浆红外光谱和拉曼光谱的影响。在这项研究中，将前列腺癌血浆样品（n = 30）分为三组（非溶血，轻度溶血，并根据溶血程度和中度溶血）使用高通量（HT）-FTIR和HT-拉曼光谱仪记录FTIR和拉曼光谱。使用主成分分析观察到了非溶血和溶血血浆样品的红外光谱和拉曼光谱之间的区别。经典最小二乘拟合分析显示非溶血和溶血血浆样品中纯组分的权重差异。因此，在比较实验内部和实验之间的结果时，有必要考虑由于溶血引起的光谱特征变化。经典最小二乘拟合分析显示非溶血和溶血血浆样品中纯组分的权重差异。因此，在比较实验内部和实验之间的结果时，有必要考虑由于溶血引起的光谱特征变化。经典最小二乘拟合分析显示非溶血和溶血血浆样品中纯组分的权重差异。因此，在比较实验内部和实验之间的结果时，有必要考虑由于溶血引起的光谱特征变化。

更新日期：2020-04-07

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