Abstract The determination of aflatoxins in kimchi is essential as it contains red pepper powder which is susceptible to aflatoxin contamination. This study was designed to determine the concentrations of aflatoxin B1, aflatoxin B2, aflatoxin G1, and aflatoxin G2 in kimchi and red pepper samples from South Korea. The aflatoxins were isolated from the samples using ultrasonic extraction in 70% methanol and an immunoaffinity column. A method for the quantification of aflatoxins in subject samples was developed and validated using ultra high-performance liquid chromatography with fluorescence detection (u-HPLC-FLD). This method showed good inter-day accuracy (86.7–112.6% recovery) and precision (5.2–11.9% relative standard deviation). The aflatoxins were analyzed in 15 samples (n = 390), with a concentration range from 0.20 to 2.33 μg/kg. In general, the aflatoxin concentrations in dried red pepper and red pepper powders were very low compared with the Korean Food Code (10 μg/kg aflatoxin B1). The analytical method was successfully applied to kimchi products for the determination of aflatoxin levels. From the results, the commercial kimchi products were concluded to be safe for human consumption with respect to aflatoxin concentrations.

中文翻译：

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超高效液相色谱荧光检测法测定红辣椒和泡菜中的黄曲霉毒素

摘要 泡菜中黄曲霉毒素含量的测定至关重要，因为泡菜中含有易受黄曲霉毒素污染的红辣椒粉。本研究旨在测定韩国泡菜和红辣椒样品中黄曲霉毒素 B1、黄曲霉毒素 B2、黄曲霉毒素 G1 和黄曲霉毒素 G2 的浓度。使用 70% 甲醇超声提取和免疫亲和柱从样品中分离出黄曲霉毒素。使用超高效液相色谱与荧光检测 (u-HPLC-FLD) 开发并验证了一种用于对受试者样品中的黄曲霉毒素进行定量的方法。该方法显示出良好的日间准确度（86.7-112.6% 的回收率）和精密度（5.2-11.9% 的相对标准偏差）。分析了 15 个样品（n = 390）中的黄曲霉毒素，浓度范围为 0.20 至 2.33 μg/kg。一般来说，与韩国食品法典（10 μg/kg 黄曲霉毒素 B1）相比，干红辣椒和红辣椒粉中的黄曲霉毒素浓度非常低。该分析方法已成功应用于泡菜产品中黄曲霉毒素含量的测定。从结果来看，商业泡菜产品在黄曲霉毒素浓度方面对人类食用是安全的。