Hydroxyapatite nanoparticles were synthesized using modified sol–gel approach. Its biocompatibility was proved by in vitro experiments with diploid fibroblasts. The experiment showed that the presence of hydroxyapatite in the environment of cells does not reduce their viability. The observed phase transition of hydroxyapatite was explained as order/disorder transition, saving the crystal structure symmetry. Organo–inorganic composites of chitosan and hydroxyapatite were obtained by preparing a joint solution and pouring onto a glass substrate. According to XRD and DTA data, addition of hydroxyapatite reduces the softening and decomposition temperature of chitosan. This information is necessary to optimize the manufacturing processes of organo–inorganic scaffolds.

中文翻译：

---

纳米羟基磷灰石-壳聚糖复合材料的理化性质研究

羟基磷灰石纳米颗粒是使用改进的溶胶-凝胶方法合成的。通过二倍体成纤维细胞的体外实验证明了其生物相容性。实验表明，细胞环境中羟基磷灰石的存在不会降低它们的生存能力。观察到的羟基磷灰石的相变被解释为有序/无序转变，从而节省了晶体结构的对称性。壳聚糖和羟基磷灰石的有机-无机复合材料是通过制备联合溶液并浇注到玻璃基板上而获得的。根据XRD和DTA数据，羟基磷灰石的加入降低了壳聚糖的软化和分解温度。这些信息对于优化有机-无机支架的制造工艺是必要的。