Metabolomics has found numerous applications in the study of liver metabolism in health and disease. Metabolomics studies can be conducted in a variety of biological matrices ranging from easily accessible biofluids such as urine, blood or feces, to organs, tissues or even cells. Sample collection and storage are critical steps for which standard operating procedures must be followed. Inappropriate sample collection or storage can indeed result in high variability, interferences with instrumentation or degradation of metabolites. In this review, we will first highlight important general factors that should be considered when planning sample collection in the study design of metabolomic studies, such as nutritional status and circadian rhythm. Then, we will discuss in more detail the specific procedures that have been described for optimal pre-analytical handling of the most commonly used matrices (urine, blood, feces, tissues and cells).

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代谢组学研究中样品采集的重要注意事项，特别关注肝功能的应用

代谢组学已在健康和疾病的肝脏代谢研究中发现了许多应用。代谢组学研究可以在多种生物基质中进行，从易于获取的生物流体（如尿液，血液或粪便）到器官，组织甚至细胞。样品的收集和存储是必须遵循标准操作程序的关键步骤。样品收集或存储不当的确会导致高度可变性，对仪器的干扰或代谢物的降解。在本文中，我们将首先重点介绍在代谢组学研究的研究设计中计划样本采集时应考虑的重要一般因素，例如营养状况和昼夜节律。然后，