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Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding.
Developmental Cell ( IF 10.7 ) Pub Date : 2020-03-12 , DOI: 10.1016/j.devcel.2020.02.012 Anthony S Eritano 1 , Claire L Bromley 1 , Antonio Bolea Albero 1 , Lucas Schütz 2 , Fu-Lai Wen 3 , Michiko Takeda 1 , Takashi Fukaya 4 , Mustafa M Sami 1 , Tatsuo Shibata 3 , Steffen Lemke 2 , Yu-Chiun Wang 1
Developmental Cell ( IF 10.7 ) Pub Date : 2020-03-12 , DOI: 10.1016/j.devcel.2020.02.012 Anthony S Eritano 1 , Claire L Bromley 1 , Antonio Bolea Albero 1 , Lucas Schütz 2 , Fu-Lai Wen 3 , Michiko Takeda 1 , Takashi Fukaya 4 , Mustafa M Sami 1 , Tatsuo Shibata 3 , Steffen Lemke 2 , Yu-Chiun Wang 1
Affiliation
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Morphological constancy is universal in developing systems. It is unclear whether precise morphogenesis stems from faithful mechanical interpretation of gene expression patterns. We investigate the formation of the cephalic furrow, an epithelial fold that is precisely positioned with a linear morphology. Fold initiation is specified by a precise genetic code with single-cell row resolution. This positional code activates and spatially confines lateral myosin contractility to induce folding. However, 20% of initiating cells are mis-specified because of fluctuating myosin intensities at the cellular level. Nevertheless, the furrow remains linearly aligned. We find that lateral myosin is planar polarized, integrating contractile membrane interfaces into supracellular "ribbons." Local reduction of mechanical coupling at the "ribbons" using optogenetics decreases furrow linearity. Furthermore, 3D vertex modeling indicates that polarized, interconnected contractility confers morphological robustness against noise. Thus, tissue-scale mechanical coupling functions as a denoising mechanism to ensure morphogenetic precision despite noisy decoding of positional information.
中文翻译：
组织规模的机械耦合降低了形态发生的噪音，以确保上皮折叠过程中的精确度。
形态恒定性在开发系统中很普遍。尚不清楚精确的形态发生是否源于对基因表达模式的忠实机械解释。我们调查头沟的形成，上皮褶皱是线性形态的精确定位。折叠起始由具有单细胞行分辨率的精确遗传密码指定。该位置代码激活并在空间上限制肌球蛋白的侧向收缩性，以诱导折叠。但是，由于在细胞水平上肌球蛋白强度的波动，错误指定了20％的起始细胞。尽管如此，犁沟仍保持线性排列。我们发现外侧肌球蛋白是平面极化的，将收缩膜界面整合到细胞上的“色带”中。在“
更新日期：2020-04-20
中文翻译：
组织规模的机械耦合降低了形态发生的噪音，以确保上皮折叠过程中的精确度。
形态恒定性在开发系统中很普遍。尚不清楚精确的形态发生是否源于对基因表达模式的忠实机械解释。我们调查头沟的形成，上皮褶皱是线性形态的精确定位。折叠起始由具有单细胞行分辨率的精确遗传密码指定。该位置代码激活并在空间上限制肌球蛋白的侧向收缩性，以诱导折叠。但是，由于在细胞水平上肌球蛋白强度的波动，错误指定了20％的起始细胞。尽管如此，犁沟仍保持线性排列。我们发现外侧肌球蛋白是平面极化的，将收缩膜界面整合到细胞上的“色带”中。在“
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