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A Biomimetic Plasmonic Nanoreactor for Reliable Metabolite Detection.
Advanced Science ( IF 14.3 ) Pub Date : 2020-03-11 , DOI: 10.1002/advs.201903730 Jiangang Liu 1 , Chenlei Cai 2 , Yuning Wang 3 , Yu Liu 1 , Lin Huang 1 , Tongtong Tian 3 , Yuanyuan Yao 3 , Jia Wei 1 , Ruoping Chen 1 , Kun Zhang 1 , Baohong Liu 3 , Kun Qian 1
Advanced Science ( IF 14.3 ) Pub Date : 2020-03-11 , DOI: 10.1002/advs.201903730 Jiangang Liu 1 , Chenlei Cai 2 , Yuning Wang 3 , Yu Liu 1 , Lin Huang 1 , Tongtong Tian 3 , Yuanyuan Yao 3 , Jia Wei 1 , Ruoping Chen 1 , Kun Zhang 1 , Baohong Liu 3 , Kun Qian 1
Affiliation
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Reliable monitoring of metabolites in biofluids is critical for diagnosis, treatment, and long-term management of various diseases. Although widely used, existing enzymatic metabolite assays face challenges in clinical practice primarily due to the susceptibility of enzyme activity to external conditions and the low sensitivity of sensing strategies. Inspired by the micro/nanoscale confined catalytic environment in living cells, the coencapsulation of oxidoreductase and metal nanoparticles within the nanopores of macroporous silica foams to fabricate all-in-one bio-nanoreactors is reported herein for use in surface-enhanced Raman scattering (SERS)-based metabolic assays. The enhancement of catalytical activity and stability of enzyme against high temperatures, long-time storage or proteolytic agents are demonstrated. The nanoreactors recognize and catalyze oxidation of the metabolite, and provide ratiometric SERS response in the presence of the enzymatic by-product H2O2, enabling sensitive metabolite quantification in a "sample in and answer out" manner. The nanoreactor makes any oxidoreductase-responsible metabolite a candidate for quantitative SERS sensing, as shown for glucose and lactate. Glucose levels of patients with bacterial infection are accurately analyzed with only 20 µL of cerebrospinal fluids, indicating the potential application of the nanoreactor in vitro clinical testing.
中文翻译：
用于可靠代谢物检测的仿生等离子体纳米反应器。
生物体液中代谢物的可靠监测对于各种疾病的诊断、治疗和长期管理至关重要。尽管广泛使用，但现有的酶代谢物测定在临床实践中面临挑战，这主要是由于酶活性对外部条件的敏感性和传感策略的低敏感性。受活细胞中微/纳米级受限催化环境的启发，本文报道了将氧化还原酶和金属纳米颗粒共封装在大孔二氧化硅泡沫的纳米孔内以制造一体式生物纳米反应器，用于表面增强拉曼散射（SERS） ）为基础的代谢测定。证明了酶对高温、长期储存或蛋白水解剂的催化活性和稳定性的增强。纳米反应器识别并催化代谢物的氧化，并在酶副产物 H2O2 存在的情况下提供比率 SERS 响应，从而以“进样和应答”的方式实现敏感的代谢物定量。纳米反应器使任何氧化还原酶响应的代谢物都成为定量 SERS 传感的候选物，如葡萄糖和乳酸所示。仅用 20 µL 脑脊液即可准确分析细菌感染患者的血糖水平，表明纳米反应器在体外临床测试中的潜在应用。
更新日期：2020-03-11
中文翻译：
用于可靠代谢物检测的仿生等离子体纳米反应器。
生物体液中代谢物的可靠监测对于各种疾病的诊断、治疗和长期管理至关重要。尽管广泛使用，但现有的酶代谢物测定在临床实践中面临挑战，这主要是由于酶活性对外部条件的敏感性和传感策略的低敏感性。受活细胞中微/纳米级受限催化环境的启发，本文报道了将氧化还原酶和金属纳米颗粒共封装在大孔二氧化硅泡沫的纳米孔内以制造一体式生物纳米反应器，用于表面增强拉曼散射（SERS） ）为基础的代谢测定。证明了酶对高温、长期储存或蛋白水解剂的催化活性和稳定性的增强。纳米反应器识别并催化代谢物的氧化，并在酶副产物 H2O2 存在的情况下提供比率 SERS 响应，从而以“进样和应答”的方式实现敏感的代谢物定量。纳米反应器使任何氧化还原酶响应的代谢物都成为定量 SERS 传感的候选物，如葡萄糖和乳酸所示。仅用 20 µL 脑脊液即可准确分析细菌感染患者的血糖水平，表明纳米反应器在体外临床测试中的潜在应用。
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