Carbon dots tailored with a fluorophore for sensitive and selective detection of hydrogen sulfide based on a ratiometric fluorescence signal,Analytical Methods

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Carbon dots tailored with a fluorophore for sensitive and selective detection of hydrogen sulfide based on a ratiometric fluorescence signal
Analytical Methods ( IF 2.7 ) Pub Date : 2020/03/11 , DOI: 10.1039/d0ay00020e
Guoqiang Liu _{1,

2,

3,

4,

5} , Hongwei Ge _{1,

2,

3,

4,

5} , Ranhao Yin _{1,

2,

3,

4,

5} , Long Yu _{1,

2,

3,

4,

5} , Congming Sun _{1,

2,

3,

4,

5} , Weiru Dong _{1,

2,

3,

4,

5} , Zhenli Sun _{1,

2,

3,

4,

5} , Khalid A. Alamry _{6,

7,

8,

9,

10} , Hadi M. Marwani _{6,

7,

8,

9,

10} , Suhua Wang _{1,

2,

3,

4,

5}

Affiliation

To discriminate hydrogen sulfide (H₂S) from thiol nucleophiles with similar reactivity is always a major challenge in developing new detection methods. In this paper, we report a colorimetric and ratiometric fluorescence probe selectively reactive to H₂S, which was synthesized by hybridizing the fluorescent nitro-benz-2-oxa-1,3-diazole (NBD)-based derivative P-NBD with carbon dots (CDs). The selective reaction can be monitored by distinct ratiometric fluorescence or colorimetric variation. The nanohybrid probe exhibited two fluorescence bands centered at 440 nm and 543 nm, respectively. The yellow fluorescence at 543 nm from P-NBD is quenched by H₂S, while the blue fluorescence is enhanced by weakening of the Förster resonance energy transfer (FRET) mechanism. It was observed that the apparent fluorescence color evolved from yellow to blue when exposed to different amounts of H₂S, accompanied by the color change of the probe solution from yellow to red under natural light. The detection limit was estimated to be 57 nM in aqueous solution. We successfully applied this probe for the detection of H₂S in real water samples, suggesting its potential application in environmental monitoring and protection.

中文翻译：

碳点量身定制的荧光基团，用于基于比例荧光信号灵敏和选择性地检测硫化氢

区分具有相似反应性的硫氢亲核试剂中的硫化氢（H ₂ S）一直是开发新检测方法的主要挑战。在本文中，我们报告了一种比色和比例荧光探针，可选择性地与H ₂ S反应，该探针是通过将基于荧光的硝基苯-2-氧杂-1,3-二唑（NBD）基衍生物P-NBD与碳杂交而合成的点（CD）。选择性反应可以通过不同的比例荧光或比色变化进行监测。纳米杂交探针显示两个分别位于440 nm和543 nm的荧光带。来自P-NBD的543 nm处的黄色荧光被H ₂淬灭S，而蓝色荧光则通过减弱Förster共振能量转移（FRET）机制来增强。观察到，当暴露于不同量的H ₂ S时，表观荧光颜色从黄色演变为蓝色，伴随着探针溶液在自然光下从黄色变为红色的颜色变化。水溶液中的检出限估计为57 nM。我们成功地将该探针用于实际水样中H ₂ S的检测，表明其在环境监测和保护中的潜在应用。

更新日期：2020-03-27

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