A mouse single taste bud contains 10-100 taste bud cells (TBCs) in which the elongated TBCs are classified into 3 cell types (types I-III) equipped with different taste receptors. Accordingly, differences in the cell numbers and ratios of respective cell types per taste bud may affect taste-nerve responsiveness. Here, we examined the numbers of each immunoreactive cell for the type II (sweet, bitter, or umami receptor cells) and type III (sour and/or salt receptor cells) markers per taste bud in the circumvallate and foliate papillae and compared these numerical features of TBCs per taste bud to those in fungiform papilla and soft palate, which we previously reported. In circumvallate and foliate taste buds, the numbers of TBCs and immunoreactive cells per taste bud increased as a linear function of the maximal cross-sectional taste bud area. Type II cells made up approximately 25% of TBCs irrespective of the regions from which the TBCs arose. In contrast, type III cells in circumvallate and foliate taste buds made up approximately 11% of TBCs, which represented almost 2 times higher than what was observed in the fungiform and soft palate taste buds. The densities (number of immunoreactive cells per taste bud divided by the maximal cross-sectional area of the taste bud) of types II and III cells per taste bud are significantly higher in the circumvallate papillae than in the other regions. The effects of these region-dependent differences on the taste response of the taste bud are discussed.

中文翻译：

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小鼠周缘和叶状味蕾中味蕾细胞数量的定量分析。

小鼠的单个味蕾包含10-100个味蕾细胞（TBC），其中细长的TBC被分为3种类型的细胞（I-III型），它们具有不同的味觉受体。因此，每个味蕾的细胞数目和每种细胞类型的比例的差异可能影响味觉神经反应性。在这里，我们检查了每个味蕾在周缘和叶状乳头中每个II型（甜味，苦味或鲜味受体细胞）和III型（酸和/或盐受体细胞）标记的免疫反应细胞的数量，并比较了这些数值每个味蕾的TBC特征与真菌状乳头和软pa中的TBC特征相同，我们之前已经报道过。在周缘和叶状味蕾中，每个味蕾的TBC和免疫反应性细胞的数目随最大横截面味蕾面积的线性函数而增加。II型细胞约占TBC的25％，而与TBC产生的区域无关。相比之下，周缘和叶状味蕾中的III型细胞约占TBC的11％，比真菌类和软taste味蕾中所观察到的高约2倍。每个味蕾的II型和III型细胞的密度（每个味蕾的免疫反应性细胞数除以味蕾的最大横截面面积）在周缘乳突中明显高于其他区域。讨论了这些区域相关的差异对味蕾的味觉响应的影响。圆形和叶状味蕾中的III型细胞约占TBC的11％，比真菌形和软pa味蕾中观察到的细胞高出近两倍。每个味蕾的II型和III型细胞的密度（每个味蕾的免疫反应性细胞数除以味蕾的最大横截面面积）在周缘乳突中明显高于其他区域。讨论了这些区域相关的差异对味蕾的味觉响应的影响。圆形和叶状味蕾中的III型细胞约占TBC的11％，比真菌形和软pa味蕾中观察到的细胞高出近两倍。每个味蕾的II型和III型细胞的密度（每个味蕾的免疫反应性细胞数除以味蕾的最大横截面面积）在周缘乳突中明显高于其他区域。讨论了这些区域相关的差异对味蕾的味觉响应的影响。每个味蕾的II型和III型细胞的密度（每个味蕾的免疫反应性细胞数除以味蕾的最大横截面面积）在周缘乳突中明显高于其他区域。讨论了这些区域相关的差异对味蕾的味觉响应的影响。每个味蕾的II型和III型细胞的密度（每个味蕾的免疫反应性细胞数除以味蕾的最大横截面面积）在周缘乳突中明显高于其他区域。讨论了这些区域相关的差异对味蕾的味觉响应的影响。