In the present study, we analyzed phenotypes of cells in the lymphocyte region of bone marrow in 68 patients with primary immune thrombocytopenia (ITP) to determine whether cellular phenotype predicts response to first-line therapy (corticosteroids or corticosteroids plus intravenous immunoglobulin). In 52 newly diagnosed ITP patients, an abnormal CD4:CD8 ratio (CD4/CD8 ratio < 0.4 and 2.3 < CD4/CD8 ratio) was noted in 22 patients in the responder group, whereas all non-responder and control individuals showed normal CD4:CD8 ratio (p < 0.001). The absolute number of CD19+ cells in patients with 0.4 ≤ CD4/CD8 ratio ≤ 2.3 or 2.3 < CD4/CD8 ratio was higher than that in other groups. (p = 0.016). In 16 chronic ITP patients, the absolute number of NK cells in the responder group was lower than those in the non-responder and control groups (p = 0.032). An abnormal CD4:CD8 ratio was noted in all patients in the responder group, whereas all patients in non-responder and control groups showed normal CD4:CD8 ratio (p < 0.001). The present results indicate that CD4:CD8 ratio, B cells, and NK cells contribute to the prediction of therapeutic outcomes of ITP patients.

中文翻译：

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通过流式细胞术分析骨髓淋巴细胞表型，预测对ITP一线治疗的反应。

在本研究中，我们分析了68例原发性免疫性血小板减少症（ITP）患者的骨髓淋巴细胞区域的细胞表型，以确定细胞表型是否可预测对一线治疗的反应（皮质类固醇或皮质类固醇加静脉内免疫球蛋白）。在52例新诊断的ITP患者中，响应者组中有22例患者发现CD4：CD8比率异常（CD4 / CD8比率<0.4和2.3 <CD4 / CD8比率），而所有无应答者和对照者均显示正常CD4： CD8比率（p <0.001）。0.4≤CD4/ CD8比≤2.3或2.3 <CD4 / CD8比的患者中CD19 +细胞的绝对数量高于其他组。（p ＝ 0.016）。在16例慢性ITP患者中，应答组中NK细胞的绝对数量低于无应答组和对照组（p = 0.032）。应答者组中所有患者的CD4：CD8比率均异常，而非应答者和对照组中的所有患者的CD4：CD8比率均正常（p <0.001）。目前的结果表明，CD4：CD8比例，B细胞和NK细胞有助于ITP患者的治疗结果的预测。