Background

MicroRNAs (miRNAs) are considered as promising cancer biomarkers. The aim of the present study is to investigate the prognostic significance of miR-200c in patients with oral squamous cell carcinoma (OSCC).

Materials and methods

Quantitative real-time PCR (qRT-PCR) was used to determine the expression levels of miR-200c in 204 pairs of OSCC and adjacent noncancerous. Correlations between miR-200c expression levels and clinicopathological characteristics were investigated. Survival analysis was performed using the Kaplan–Meier method and log-rank test. Multivariate analysis of the prognostic factors was performed with a Cox proportional hazards regression model.

Results

The expression of miR-200c was significantly down-regulated in OSCC tissues compared with adjacent non-tumor tissues (p < 0.0001). Low expression of miR-200c in tumor tissues was significantly correlated with the positive N classification (p = 0.013), advanced TNM stage (p = 0.007) and poor differentiation grade (p = 0.026). Lower miR-200c expression in patients was significantly associated with poor recurrence-free survival (RFS, p = 0.0003) and overall survival (OS, p = 0.0026). Multivariate analysis confirmed that low miR-200c expression was an independent predictor for poor RFS (hazard ratio (HR) 1.705, 95% CI 1.136–2.56, p = 0.01) and OS (HR 1.669, 95% CI 1.03–2.703, p = 0.037) in patients with OSCC.

Conclusion

Our results suggest that the miR-200c might be a potential prognostic biomarker for OSCC.

中文翻译：

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miR-200c 的下调与口腔鳞状细胞癌的不良预后相关。

背景

MicroRNAs (miRNAs) 被认为是有前途的癌症生物标志物。本研究的目的是研究 miR-200c 在口腔鳞状细胞癌 (OSCC) 患者中的预后意义。

材料和方法

定量实时 PCR (qRT-PCR) 用于确定 204 对 OSCC 和相邻非癌中 miR-200c 的表达水平。研究了 miR-200c 表达水平与临床病理特征之间的相关性。使用 Kaplan-Meier 方法和对数秩检验进行生存分析。使用 Cox 比例风险回归模型对预后因素进行多变量分析。

结果

与邻近的非肿瘤组织相比，OSCC 组织中 miR-200c 的表达显着下调（p  < 0.0001）。miR-200c在肿瘤组织中的低表达与N 分期阳性（p =0.013）、TNM晚期（p  =0.007）和分化程度差（p  =0.026）显着相关。患者中较低的 miR-200c 表达与较差的无复发生存率（RFS，p  = 0.0003）和总生存率（OS，p  = 0.0026）显着相关。多变量分析证实，低 miR-200c 表达是不良 RFS 的独立预测因子（风险比 (HR) 1.705, 95% CI 1.136–2.56, p = 0.01) 和 OS（HR 1.669，95% CI 1.03–2.703，p  = 0.037）在 OSCC 患者中。

结论

我们的结果表明 miR-200c 可能是 OSCC 的潜在预后生物标志物。