Nitric oxide (NO) is related to a wide range of physiological processes such as vasodilation, macrophages cytotoxicity and wound healing. The human skin contains NO precursors (NO_x). Those are mainly composed of nitrite (NO₂⁻), nitrate (NO₃⁻), and S-nitrosothiols (RSNOs) which forms a large NO store. These NO_x stores in human skin can mobilize NO to blood stream upon ultraviolet (UV) light exposure. The main purpose of this study was to evaluate the most effective UV light wavelength to generate NO and compare it to each NO precursor in aqueous solution. In addition, the UV light might change the RSNO content on human skin. First, we irradiated pure aqueous solutions of NO₂⁻ and NO₃⁻ and mixtures of NO₂⁻ and glutathione and NO₃⁻ and S-nitrosoglutathione (GSNO) to identify the NO release profile from those species alone. In sequence, we evaluated the NO generation profile on human skin slices. Human skin was acquired from redundant plastic surgical samples and the NO and RSNO measurements were performed using a selective NO electrochemical sensor. The data showed that UV light could trigger the NO generation in skin with a peak at 280–285 nm (UVB range). We also observed a significant RSNO formation in irradiated human skin, with a peak at 320 nm (UV region) and at 700 nm (visible region). Pre-treatment of the human skin slice using NO₂⁻ and thiol (RSHs) scavengers confirmed the important role of these molecules in RSNO formation. These findings have important implications for clinical trials with potential for new therapies.

一氧化氮 (NO) 与多种生理过程有关，例如血管舒张、巨噬细胞的细胞毒性和伤口愈合。人体皮肤不含任何前体（NO _x ）。它们主要由亚硝酸盐（NO ₂ ^- ）、硝酸盐（NO ₃ ^- ）和S-亚硝基硫醇（RSNO）组成，形成大量的NO储存。人类皮肤中储存的这些 NO _x可以在紫外线 (UV) 光照射下将 NO 调动到血流中。本研究的主要目的是评估产生 NO 的最有效紫外光波长，并将其与水溶液中的每种 NO 前体进行比较。此外，紫外线可能会改变人体皮肤上RSNO的含量。首先，我们照射了 NO ₂ ^-和 NO ₃ ^-的纯水溶液以及 NO ₂ ^-和谷胱甘肽以及 NO ₃ ^-和S-亚硝基谷胱甘肽 (GSNO) 的混合物，以识别这些物种单独的 NO 释放曲线。接下来，我们评估了人体皮肤切片上 NO 的生成情况。人体皮肤取自多余的整形手术样本，并使用选择性 NO 电化学传感器进行 NO 和 RSNO 测量。数据显示，紫外线可以触发皮肤中一氧化氮的生成，峰值位于 280-285 nm（UVB 范围）。我们还在受辐射的人体皮肤中观察到显着的 RSNO 形成，峰值位于 320 nm（紫外线区域）和 700 nm（可见光区域）。使用 NO ₂ ^-和硫醇 (RSH) 清除剂对人体皮肤切片进行预处理，证实了这些分子在 RSNO 形成中的重要作用。 这些发现对于具有新疗法潜力的临床试验具有重要意义。