This study aimed at assessment of the long-term (4 weeks) metabolic effect of a diet with and without beetroot juice supplementation in fencers using the untargeted metabolomics method with the UPLC Q-TOF/MS system to carry out an analysis of urine samples. Ten women and 10 men underwent the cardiovascular fitness VO_2max test at baseline—(B) and after two stages of implementation of the dietary recommendations—the first 4 weeks without beetroot juice (D) and the second with 26 g/d of freeze-dried beetroot juice supplementation (D&J). The urine samples were collected one hour after the VO_2max test at B and after D and D&J. The meal before the VO_2max test after D&J contained beetroot juice, whereas to the meal at B and after D maltodextrin was added. Changes in metabolites and VO_2max were significant only for comparison of D versus D&J. During D and D&J, there were no significant changes in the physical activity level, body mass, and body composition. We observed significant changes in tyrosine and tryptophan metabolism, mainly associated with such neurotransmitter’s metabolism as: Serotonin, noradrenaline, and adrenaline. Changes in signal intensity of bile acid, AICAR, and 4-Hydroxynonenal (peroxidation of polyunsaturated fatty acids product) were also observed. The obtained results indicate that long-term beetroot juice supplementation induces considerable changes in metabolism.

中文翻译：

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研究击剑者补充甜菜根汁代谢作用的无目标代谢组学方法-初步研究

这项研究旨在通过使用UPLC Q-TOF / MS系统的非靶向代谢组学方法评估在围篱中添加和不添加甜菜根汁的饮食的长期（4周）代谢效果，以分析尿液样品。10名妇女和10名男性在基线时进行了心血管适应性VO _2max测试（B），并且在实施了饮食建议的两个阶段之后进行了测试-前4周未使用甜菜根汁（D），第二周进行了26 g / d的冷冻-干甜菜根汁补充剂（D＆J）。VO _2max测试后一小时，在B以及D和D＆J之后收集尿液样本。VO _2max之前的饭D＆J之后的测试包含甜菜根汁，而B和D之后的膳食中添加了麦芽糊精。代谢物和VO _2max的变化仅对于D与D＆J的比较才有意义。在D和D＆J期间，身体活动水平，体重和身体组成均无明显变化。我们观察到酪氨酸和色氨酸代谢的显着变化，主要与以下神经递质的代谢有关：5-羟色胺，去甲肾上腺素和肾上腺素。还观察到了胆汁酸，AICAR和4-Hydroxynonenal（多不饱和脂肪酸产物的过氧化）信号强度的变化。获得的结果表明，长期补充甜菜根汁会引起新陈代谢的显着变化。