Cell-laden hydrogels are widely used in tissue engineering and regenerative medicine. However, many of these hydrogels are not optimized for use in the oral environment, where they are exposed to blood and saliva. To address these challenges, we engineered an alginate-based adhesive, photocrosslinkable, and osteoconductive hydrogel biomaterial (AdhHG) with tunable mechanical properties. The engineered hydrogel was used as an injectable mesenchymal stem cell (MSC) delivery vehicle for craniofacial bone tissue engineering applications. Subcutaneous implantation in mice confirmed the biodegradability, biocompatibility, and osteoconductivity of the hydrogel. In a well-established rat peri-implantitis model, application of the adhesive hydrogel encapsulating gingival mesenchymal stem cells (GMSCs) resulted in complete bone regeneration around ailing dental implants with peri-implant bone loss. Together, we have developed a distinct bioinspired adhesive hydrogel with tunable mechanical properties and biodegradability that effectively delivers patient-derived dental-derived MSCs. The hydrogel is photocrosslinkable and, due to the presence of MSC aggregates and hydroxyapatite microparticles, promotes bone regeneration for craniofacial tissue engineering applications.

载有细胞的水凝胶广泛用于组织工程和再生医学。但是，这些水凝胶中有许多并未针对在暴露于血液和唾液的口腔环境中进行优化。为了解决这些挑战，我们设计了一种基于藻酸盐的粘合剂，可光交联的骨传导性水凝胶生物材料（AdhHG），其机械性能可调。工程水凝胶用作颅面骨组织工程应用的可注射间充质干细胞（MSC）传递载体。小鼠皮下植入证实了水凝胶的生物降解性，生物相容性和骨传导性。在成熟的大鼠种植体周围炎模型中，粘合性水凝胶封装牙龈间充质干细胞（GMSC）的应用导致患病的牙科植入物周围完全骨再生，而植入物周围骨质流失。我们共同开发了独特的具有生物启发性的粘合水凝胶，具有可调节的机械性能和可生物降解性，可有效递送患者来源的牙科来源的MSC。水凝胶是可光交联的，由于存在MSC聚集体和羟基磷灰石微粒，可促进颅面组织工程应用中的骨再生。