当前位置: X-MOL 学术J. Electroanal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A capacitive immunosensor for tetracycline estimation using antibody modified polytyramine-alkanethiol ultra-thin film on gold
Journal of Electroanalytical Chemistry ( IF 4.1 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.jelechem.2020.114055
Arun Kumar Prusty , Sunil Bhand

Abstract A Polytyramine-alkanethiol based electrochemical capacitive immunosensor was developed for tetracycline (TC) antibiotic detection in water. Tyramine (TA) monomer was electropolymerized to get an insulating ultrathin polytyramine (PTA) film on the gold-coated silicon electrode (AuE). The PTA coated AuEs were incubated in 1-Dodecanethiol to get thiol filled polymer-coated electrodes. This process of thiolation filled the pinholes of the polymer surface and enhanced the insulation of the polymer electrode, suited for capacitive sensor development. The thiolated polymer electrodes were modified with polyclonal antibodies against TC to get TC immunosensor (TCI). The prepared TCI was characterized at various steps of fabrication. The surface characterization was performed by using an atomic force microscope (AFM). The electrochemical characterizations were performed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) technique. Further, the EIS measurements were carried out to measure the capacitance change of TCI in the presence of TC in matrix-matched water samples. The fabricated TCI exhibited linearity in 1 to 200 μgL−1 range for TC, with a limit of detection 0.01 μgL−1. Further, the fabricated TCI exhibited good selectivity towards TC with recovery from 96 to 102% in spiked water samples. Results indicate the possible application of developed immunosensor for TC estimation in water samples.

中文翻译:

使用抗体修饰的聚酪胺-烷硫醇超薄膜在金上进行四环素估计的电容免疫传感器

摘要 开发了一种基于聚酪胺-烷硫醇的电化学电容免疫传感器,用于检测水中的四环素 (TC) 抗生素。酪胺 (TA) 单体被电聚合以在镀金硅电极 (AuE) 上获得绝缘的超薄聚酪胺 (PTA) 膜。将 PTA 涂层的 AuEs 在 1-十二烷硫醇中培养以获得硫醇填充的聚合物涂层电极。这种硫醇化过程填充了聚合物表面的针孔并增强了聚合物电极的绝缘性,适用于电容传感器的开发。用抗TC的多克隆抗体修饰硫醇化聚合物电极以获得TC免疫传感器(TCI)。制备的 TCI 在不同的制造步骤中进行了表征。通过使用原子力显微镜(AFM)进行表面表征。通过循环伏安法(CV)和电化学阻抗谱(EIS)技术进行电化学表征。此外,还进行了 EIS 测量以测量基质匹配水样中存在 TC 时 TCI 的电容变化。制备的 TCI 在 1 到 200 μgL-1 范围内对 TC 表现出线性,检测限为 0.01 μgL-1。此外,制备的 TCI 对 TC 表现出良好的选择性,在加标水样品中的回收率为 96% 至 102%。结果表明开发的免疫传感器可能应用于水样中的 TC 估计。进行 EIS 测量是为了测量基质匹配水样中存在 TC 时 TCI 的电容变化。制备的 TCI 在 1 到 200 μgL-1 范围内对 TC 表现出线性,检测限为 0.01 μgL-1。此外,制备的 TCI 对 TC 表现出良好的选择性,在加标水样品中的回收率为 96% 至 102%。结果表明开发的免疫传感器可能应用于水样中的 TC 估计。进行 EIS 测量是为了测量基质匹配水样中存在 TC 时 TCI 的电容变化。制备的 TCI 在 1 到 200 μgL-1 范围内对 TC 表现出线性,检测限为 0.01 μgL-1。此外,制备的 TCI 对 TC 表现出良好的选择性,在加标水样品中的回收率为 96% 至 102%。结果表明开发的免疫传感器可能应用于水样中的 TC 估计。
更新日期:2020-04-01
down
wechat
bug