Abstract Encapsulation of probiotic bacteria requires precise control over a range of process parameters, all of which are technique-specific. The technology of encapsulation has received considerable attention in recent years, owing to the numerous ranges of benefits it offers, particularly in terms of quality retention, storage stability, and targeted delivery. In this research, spray-drying (SD) and spray-freeze-drying (SFD) techniques were used to encapsulate Lactobacillus plantarum (NCIM 2083). Fructo-oligosaccharide (FOS), whey protein (WP) and maltodextrin (MD) were used as encapsulating agents at different formulations. Changes in physicochemical properties were evaluated. FOS:WP:MD (2:0.5:0.5) gave higher encapsulation efficiencies of around 89.21% and 96.16% for SD and SFD, respectively. Probiotic stability was studied under simulated in-vitro digestion conditions and over the storage period of 60 days. SFD synbiotics showed better survivability during digestion; whereas, storage studies indicated around 0.95 fold increased retention of cell viability in SD synbiotics as compared to SFD.

中文翻译：

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包封方法对合生素粉末中植物乳杆菌 (NCIM, 2083) 理化性质和稳定性的影响和体外消化条件

摘要 益生菌的封装需要对一系列工艺参数进行精确控制，所有这些参数都是特定于技术的。近年来，封装技术受到了相当多的关注，因为它提供了许多好处，特别是在质量保持、储存稳定性和有针对性的交付方面。在这项研究中，喷雾干燥 (SD) 和喷雾冷冻干燥 (SFD) 技术用于封装植物乳杆菌 (NCIM 2083)。低聚果糖 (FOS)、乳清蛋白 (WP) 和麦芽糊精 (MD) 被用作不同配方的包封剂。评估物理化学性质的变化。FOS:WP:MD (2:0.5:0.5) 分别为 SD 和 SFD 提供了更高的封装效率，分别约为 89.21% 和 96.16%。在模拟体外消化条件和 60 天的储存期内研究了益生菌的稳定性。SFD合生元在消化过程中表现出更好的生存能力；而储存研究表明，与 SFD 相比，SD 合生元中细胞活力的保留增加了约 0.95 倍。