Abstract The objective of this study was to evaluate the antagonism of Clonostachys rosea LQC62 and Trichoderma asperellum LQC96 against Botrytis cinerea and Myrothecium roridum, and to evaluate the application of these antagonists in the management of the diseases caused by these pathogens in commercial cultivation. Under controlled in vitro conditions, the production of soluble and volatile compounds was evaluated along with parasitism in paired cultures, competition by colonization in begonia leaf discs, and the effect of the application of the antagonists in the control of gray mold and Myrothecium leaf spot in the commercial cultivation of begonia. Both LQC62 and LQC96 produced soluble compounds in a culture medium that were capable of inhibiting the development of B. cinerea, which was not observed for M. roridum. Both antagonists produced volatile compounds that led to a reduction in the development of the pathogens. There was an overlap of the colonies of the antagonists with those of the pathogens, indicating hyper-parasitic activity. The antagonists colonized the begonia leaf discs, and the application of these antagonists prior to inoculation promoted a reduction in the sporulation of the pathogens of up to 100%. Weekly foliar sprays of LQC62 reduced the incidence of gray mold and of Myrothecium leaf spot in commercial cultivation.

中文翻译：

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秋海棠灰霉病和黑斑病的生物防治

摘要 本研究的目的是评价Clonostachys rosea LQC62 和Trichoderma asperellum LQC96 对Botrytis cinerea 和Myrothecium roridum 的拮抗作用，并评估这些拮抗剂在商业栽培中管理这些病原体引起的疾病中的应用。在受控的体外条件下，评估可溶性和挥发性化合物的产生以及配对培养中的寄生性、秋海棠叶盘中的定植竞争以及应用拮抗剂在控制灰霉病和漆斑病斑病中的效果。秋海棠的商业化种植。LQC62 和 LQC96 都在培养基中产生了可溶性化合物，它们能够抑制 B. cinerea 的发育，而这在 M. roridum 中没有观察到。两种拮抗剂都会产生挥发性化合物，从而减少病原体的发展。拮抗剂的菌落与病原体的菌落重叠，表明存在超寄生活性。拮抗剂定植于秋海棠叶盘上，接种前应用这些拮抗剂可将病原体的孢子形成减少多达 100%。LQC62 的每周叶面喷洒降低了商业栽培中灰霉病和漆斑霉病的发生率。并且在接种前应用这些拮抗剂可将病原体的孢子形成减少多达 100%。LQC62 的每周叶面喷洒降低了商业栽培中灰霉病和漆斑霉病的发生率。并且在接种前应用这些拮抗剂可将病原体的孢子形成减少多达 100%。LQC62 的每周叶面喷洒降低了商业栽培中灰霉病和漆斑霉病的发生率。