The potato tuber moth (PTM), Phthorimaea operculella (Zeller), is a worldwide pest that feeds on both the leaves and tubers of potato plants. PTM larvae can digest leaves, or tubers, resulting in serious damage to potato plants in the field and potato tubers in storage. To understand how midgut bacterial diversity is influenced by the consumption of these two tissue types, the symbiotic bacteria in the potato-feeding PTM midgut and the endophytic bacteria of potato tissues were analyzed. At the genus level, the bacterial community composition in the PTM midgut was influenced by the tissues consumed, owing to their different nutrient contents. Escherichia_Shigella and Enterobacter were the most dominant genera in the midgut of leaf-feeding and tuber-feeding PTMs, respectively. Interestingly, even though only present in low abundance in leaves and tubers, Escherichia_Shigella were dominantly distributed only in the midgut of leaf-feeding PTMs, indicating that specific accumulation of these genera have occurred by feeding on leaves. Moreover, Enterobacter, the most dominant genus in the midgut of tuber-feeding PTMs, was undetectable in all potato tissues, indicating it is gut-specific origin and tuber feeding-specific accumulation. Both Escherichia_Shigella and Enterobacter abundances were positively correlated with the dominant contents of potato leaves and tubers, respectively. Enrichment of specific PTM midgut bacterial communities was related to different nutrient levels in different tissues consumed by the insect, which in turn influenced host utilization. We provide evidence that a portion of the intestinal microbes of PTMs may be derived from potato endophytic bacteria and improve the understanding of the relationship between potato endophytic bacteria and the gut microbiota of PTMs, which may offer support for integrated management of this worldwide pest.

中文翻译：

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与食用马铃薯组织有关的马铃薯块茎蛾中肠微生物群多样性。

马铃薯块茎蛾（PTM），即马铃薯（Pellermanea operculella，Zeller）是一种世界性害虫，以马铃薯的叶子和块茎为食。PTM幼虫可以消化叶子或块茎，从而严重损害田间马铃薯植株和储存的马铃薯块茎。为了了解中肠细菌多样性如何受这两种组织类型的消耗影响，分析了以马铃薯为食的PTM中肠中的共生细菌和马铃薯组织的内生细菌。在属水平上，由于营养成分不同，PTM中肠中细菌群落的组成受到所消耗组织的影响。大肠埃希菌和志贺氏菌分别是以叶为食和以块茎为食的PTMs中肠中最主要的属。有趣的是 尽管仅在叶片和块茎中丰度较低，但埃希氏菌仅在以叶片饲喂的PTM的中肠中占优势，这表明这些属通过以叶片为食进行了特定的积累。此外，在所有马铃薯组织中均未检出以块茎为食的PTMs中肠中最主要的属肠杆菌，这是肠道特异性起源和块茎以食性积累。大肠杆菌和志贺氏菌的丰度分别与马铃薯叶片和块茎的主要含量呈正相关。特定PTM中肠细菌群落的富集与昆虫消耗的不同组织中不同的营养水平有关，进而影响了宿主的利用。