CT/MR Dual-Modality Imaging Tracking of Mesenchymal Stem Cells Labeled with a Au/GdNC@SiO2 Nanotracer in Pulmonary Fibrosis,ACS Applied Bio Materials

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CT/MR Dual-Modality Imaging Tracking of Mesenchymal Stem Cells Labeled with a Au/GdNC@SiO2 Nanotracer in Pulmonary Fibrosis
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-03-11 , DOI: 10.1021/acsabm.0c00195
Jie Huang ₁ , Jie Holly Huang ₂ , Hongying Bao ₁ , Xinyu Ning ₁ , Chenggong Yu ₁ , Zhongjin Chen ₁ , Jie Chao ₂ , Zhijun Zhang ₁

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Mesenchymal stem cells (MSCs) have shown potential as an innovative treatment for pulmonary fibrosis (PF), due to their capability to ameliorate the inflammation and moderate the deterioration of PF. The fate of the stem cells transplanted into the lung, including survival, migration, homing, and functions, however, has not been fully understood yet. In this paper, we report the development of a computed tomography/magnetic resonance (CT/MR) dual-modal nanotracer, gold/gadolinium nanoclusters overcoated with a silica shell (Au/GdNC@SiO₂), for noninvasive labeling and tracking of the transplanted human MSCs (hMSCs) in a PF model. The Au/GdNC@SiO₂ nanotracer exhibits good colloidal and chemical stability, high biocompatibility, enhanced longitudinal MR relaxivity, and superior X-ray attenuation property. The hMSCs can be effectively labeled with Au/GdNC@SiO₂, resulting in a significantly increased cellular CT/MR imaging contrast, without any obvious adverse effect on the function, including proliferation and differentiation of the labeled stem cells. Moreover, by using the Au/GdNC@SiO₂ nanotracer, the hMSCs transplanted in the lung can be tracked for 7 d via in vivo CT/MR dual-modality imaging. This work may provide an insight into the role the transplanted hMSCs play in PF therapy, thus promoting the stem cell-based regenerative medicine.

中文翻译：

肺纤维化中用 Au/GdNC@SiO2 纳米示踪剂标记的间充质干细胞的 CT/MR 双模态成像跟踪

间充质干细胞 (MSCs) 已显示出作为肺纤维化 (PF) 创新疗法的潜力，因为它们具有改善炎症和缓解 PF 恶化的能力。然而，移植到肺部的干细胞的命运，包括存活、迁移、归巢和功能，尚未完全清楚。在本文中，我们报告了计算机断层扫描/磁共振 (CT/MR) 双模式纳米示踪剂的开发，金/钆纳米团簇覆盖有二氧化硅壳 (Au/GdNC@SiO ₂ )，用于无创标记和跟踪在 PF 模型中移植人类 MSCs (hMSCs)。Au/GdNC@SiO ₂纳米示踪剂具有良好的胶体和化学稳定性、高生物相容性、增强的纵向磁共振弛豫性和优异的 X 射线衰减特性。_{用Au/GdNC@SiO 2}可以有效地标记hMSCs ，导致细胞CT/MR成像对比度显着增加，对功能没有任何明显的不利影响，包括标记的干细胞的增殖和分化。此外，通过使用Au/GdNC@SiO ₂纳米示踪剂，可以通过体内CT/MR双模态成像跟踪移植到肺中的hMSCs 7 d。这项工作可以深入了解移植的 hMSC 在 PF 治疗中的作用，从而促进基于干细胞的再生医学。

更新日期：2020-03-11

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