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Glutamine and methionine targeted pulsed electric field treatment for enhanced immune activity in pine nut Gln‐Trp‐Phe‐Met peptides
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-03-09 , DOI: 10.1111/ijfs.14558 LiangZi Sun 1 , Shuyu Zhang 1 , Liyan Yuan 1 , Yiying Yang 1 , Songyi Lin 1
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-03-09 , DOI: 10.1111/ijfs.14558 LiangZi Sun 1 , Shuyu Zhang 1 , Liyan Yuan 1 , Yiying Yang 1 , Songyi Lin 1
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The immunocompetence of an immune regulatory active Gln‐Trp‐Phe‐Met (QWFM) tetrapeptide obtained from pine nut protein was enhanced using a pulsed electric field (PEF). The effects of PEF were estimated based on phagocytosis and nitric oxide (NO) assays of RAW 264.7 macrophage cells. PEF significantly (P < 0.05) increased the ability of the macrophages to perform neutral red phagocytosis and produce NO. The relationship between the activity and structure of the peptide was further investigated using infrared, Raman, circular dichroism (CD) and one‐dimensional nuclear magnetic resonance spectroscopies. The infrared and Raman spectra indicated that the primary structure and functional groups of the peptide were not altered by PEF treatment, while the CD revealed that the secondary structures of the peptide were affected. Furthermore, PEF treatment changed the chemical environment of the active hydrogen protons. The PEF treatment targeted the glutamine (Q) and methionine (M) in the QWFM tetrapeptide, both of which were specifically related to the immunological activity of the peptide. This overall enhancement of QWFM immunoregulatory activity was attributed to the changes in the spatial force and the state in the peptide solution system.
中文翻译：
谷氨酰胺和蛋氨酸靶向脉冲电场处理可增强松子Gln-Trp-Phe-Met肽的免疫活性
使用脉冲电场（PEF）可增强从松子仁蛋白中获得的具有免疫调节活性的Gln-Trp-Phe-Met（QWFM）四肽的免疫能力。PEF的影响是根据RAW 264.7巨噬细胞的吞噬作用和一氧化氮（NO）分析估算的。PEF显着（P <0.05）增强了巨噬细胞执行中性红色吞噬作用并产生NO的能力。使用红外，拉曼，圆二色性（CD）和一维核磁共振波谱进一步研究了肽的活性与结构之间的关系。红外和拉曼光谱表明，PEF处理不会改变肽的一级结构和官能团，而CD则表明该肽的二级结构受到影响。此外，PEF处理改变了活性氢质子的化学环境。PEF处理针对QWFM四肽中的谷氨酰胺（Q）和蛋氨酸（M），两者均与该肽的免疫活性特别相关。
更新日期：2020-03-09
中文翻译：
谷氨酰胺和蛋氨酸靶向脉冲电场处理可增强松子Gln-Trp-Phe-Met肽的免疫活性
使用脉冲电场（PEF）可增强从松子仁蛋白中获得的具有免疫调节活性的Gln-Trp-Phe-Met（QWFM）四肽的免疫能力。PEF的影响是根据RAW 264.7巨噬细胞的吞噬作用和一氧化氮（NO）分析估算的。PEF显着（P <0.05）增强了巨噬细胞执行中性红色吞噬作用并产生NO的能力。使用红外，拉曼，圆二色性（CD）和一维核磁共振波谱进一步研究了肽的活性与结构之间的关系。红外和拉曼光谱表明，PEF处理不会改变肽的一级结构和官能团，而CD则表明该肽的二级结构受到影响。此外，PEF处理改变了活性氢质子的化学环境。PEF处理针对QWFM四肽中的谷氨酰胺（Q）和蛋氨酸（M），两者均与该肽的免疫活性特别相关。
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