A simple and ultrasensitive flow injection chemiluminescence competitive immunoassay based on gold nanoparticle‐loaded enzyme for the detection of chloramphenicol (CAP) residues in shrimp and honey has been developed. Due to their good biocompatibility and large specific surface area, carboxylic resin beads can be used as solid phase carriers to immobilize more coating antigens (Ag). In addition, gold nanoparticles could provide an effective matrix for loading more CAP antibody and horseradish peroxidase, which would effectively catalyze the system of luminol–p‐iodophenol (PIP)–H₂O₂. A competitive immunoassay strategy was used for detection of CAP, in which CAP in the sample would compete with the coating Ag for the limited antibodies, leading to a chemiluminescence (CL) signal decrease with increase in CAP concentration. A wide linear range 0.001–10 ng ml⁻¹ (R² = 0.9961) was obtained under optimized conditions, and the detection limit (3σ) was calculated to be 0.33 pg ml⁻¹. This method was also been successfully applied to determine CAP in shrimp and honey samples. The immunosensor proposed in this study not only has the advantages of high sensitivity, wider linear range, and satisfactory stability, but also expands the application of flow injection CL immunoassay in antibiotic detection.

中文翻译：

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一种基于金纳米粒子负载酶的氯霉素简单，灵敏的流动注射化学发光免疫分析方法。

一种基于金纳米颗粒负载酶的简单，超灵敏的流动注射化学发光竞争性免疫分析方法，用于检测虾和蜂蜜中的氯霉素（CAP）残留。由于其良好的生物相容性和较大的比表面积，羧酸树脂珠可以用作固相载体以固定更多的包被抗原（Ag）。此外，金纳米粒子可以提供一个有效的基质，以装载更多的CAP抗体和辣根过氧化物酶，这将有效催化鲁米诺–对碘代酚（PIP）–H ₂ O _{2的系统。}。竞争性免疫分析策略用于检测CAP，其中样品中的CAP将与包被Ag竞争有限的抗体，导致化学发光（CL）信号随CAP浓度的增加而降低。在优化条件下获得了宽线性范围0.001–10 ng ml ^-1（R ² = 0.9961），计算出的检出限（3σ）为0.33 pg ml ^-1。该方法也已成功地用于测定虾和蜂蜜样品中的CAP。本研究提出的免疫传感器不仅具有灵敏度高，线性范围宽，稳定性好等优点，而且扩大了流动注射CL免疫测定在抗生素检测中的应用。