To turn on the fluorescence of the native green fluorescence protein (GFP) chromophore, 4-hydroxybenzylidene-dimethylimidazolinone (HBDI), in an artificial supramolecular system has been a challenging task, because it requires high local environmental rigidity. This work shows that the formation of H-aggregates of an HBDI-containing organogelator results in two orders of magnitude fluorescence enhancement (Φf =2.9 vs. 0.02 %), in which the inter-HBDI OH⋅⋅⋅OH H-bonds play a crucial role. The aggregation-induced fluorescence enhancement of HBDI has important implications on the origin of the high fluorescence quantum efficiency of HBDI in the GFP β-barrel and on the supramolecular strategy for a full fluorescence recovery of HBDI. These results reveal a new approach to designing rigid chromophore aggregates for high-performance optoelectronic properties.

中文翻译：

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氢键诱导的H聚集的GFP发色团的荧光开启：超分子结构刚性。

在人工超分子系统中，要打开天然绿色荧光蛋白（GFP）生色团4-羟基苄叉基-二甲基咪唑啉酮（HBDI）的荧光是一项艰巨的任务，因为它需要很高的局部环境刚性。这项工作表明，含HBDI的有机胶凝剂的H聚集体的形成导致荧光增强两个数量级（Φf= 2.9 vs. 0.02％），其中HBDI的OH⋅⋅⋅OHH相互键关键的角色。聚集诱导的HBDI荧光增强对GFPβ-桶中HBDI的高荧光量子效率的起源以及HBDI完全荧光回收的超分子策略具有重要意义。