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Fucoxanthin alleviates palmitate-induced inflammation in RAW 264.7 cells through improving lipid metabolism and attenuating mitochondrial dysfunction.
Food & Function ( IF 5.1 ) Pub Date : 2020-03-10 , DOI: 10.1039/d0fo00442a Siyu Li 1 , Xiaomeng Ren 1 , Yuandong Wang 1 , Jiangning Hu 1 , Haitao Wu 1 , Shuang Song 1 , Chunhong Yan 2
Food & Function ( IF 5.1 ) Pub Date : 2020-03-10 , DOI: 10.1039/d0fo00442a Siyu Li 1 , Xiaomeng Ren 1 , Yuandong Wang 1 , Jiangning Hu 1 , Haitao Wu 1 , Shuang Song 1 , Chunhong Yan 2
Affiliation
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In this study, we aimed to examine the effects of fucoxanthin on inflammation triggered by palmitate in macrophages. Raw 264.7 cells were treated with palmitate with or without fucoxanthin co-treatment. Fucoxanthin greatly alleviated palmitate-induced decrease in cell viability and loss of mitochondrial membrane potential. Fucoxanthin also significantly attenuated the palmitate-induced transcriptional expression of Il-6, Il-1β, Tnfα and Nlrp3 inflammasomes and increased the expression of Tgfb. In addition, fucoxanthin decreased triglyceride accumulation induced by palmitate through enhancing the expression of Cpt1a, Pparg and other lipid metabolism genes. Inhibition of CPT1a by etomoxir attenuated the anti-inflammatory effect of fucoxanthin. Furthermore, fucoxanthin increased AMPK phosphorylation and AMPKa1 knockdown by its specific siRNA diminished protective function. In addition, fucoxanthin restored palmitate-mediated mitochondrial dysfunction and improved mitophagy-related gene expression. These findings suggest that fucoxanthin could attenuate free fatty acid-induced inflammation in macrophages through modulating lipid metabolism and mitigating mitochondrial dysfunction.
中文翻译：
岩藻黄质通过改善脂质代谢和减轻线粒体功能障碍，减轻了RAW 264.7细胞中棕榈酸酯诱导的炎症。
在这项研究中，我们旨在检查岩藻黄质对巨噬细胞棕榈酸酯引发的炎症的影响。用或不使用岩藻黄质共同处理的棕榈酸酯处理未加工的264.7细胞。岩藻黄质极大地缓解了棕榈酸酯诱导的细胞活力下降和线粒体膜电位丧失。岩藻黄质还显着减弱棕榈酸酯诱导的Il-6，Il-1β，Tnfα和Nlrp3炎性小体的转录表达，并增加Tgfb的表达。此外，岩藻黄质通过增强Cpt1a，Pparg和其他脂质代谢基因的表达，减少了棕榈酸酯诱导的甘油三酸酯积累。依托莫西对CPT1a的抑制作用减弱了岩藻黄质的抗炎作用。此外，岩藻黄质通过其特异的siRNA增加了AMPK磷酸化和AMPKa1敲低，从而降低了保护功能。此外，岩藻黄质还恢复了棕榈酸酯介导的线粒体功能障碍，并改善了线粒体相关基因的表达。这些发现表明，岩藻黄质可以通过调节脂质代谢和减轻线粒体功能障碍来减轻巨噬细胞中游离脂肪酸诱导的炎症。
更新日期：2020-03-10
中文翻译：
岩藻黄质通过改善脂质代谢和减轻线粒体功能障碍，减轻了RAW 264.7细胞中棕榈酸酯诱导的炎症。
在这项研究中，我们旨在检查岩藻黄质对巨噬细胞棕榈酸酯引发的炎症的影响。用或不使用岩藻黄质共同处理的棕榈酸酯处理未加工的264.7细胞。岩藻黄质极大地缓解了棕榈酸酯诱导的细胞活力下降和线粒体膜电位丧失。岩藻黄质还显着减弱棕榈酸酯诱导的Il-6，Il-1β，Tnfα和Nlrp3炎性小体的转录表达，并增加Tgfb的表达。此外，岩藻黄质通过增强Cpt1a，Pparg和其他脂质代谢基因的表达，减少了棕榈酸酯诱导的甘油三酸酯积累。依托莫西对CPT1a的抑制作用减弱了岩藻黄质的抗炎作用。此外，岩藻黄质通过其特异的siRNA增加了AMPK磷酸化和AMPKa1敲低，从而降低了保护功能。此外，岩藻黄质还恢复了棕榈酸酯介导的线粒体功能障碍，并改善了线粒体相关基因的表达。这些发现表明，岩藻黄质可以通过调节脂质代谢和减轻线粒体功能障碍来减轻巨噬细胞中游离脂肪酸诱导的炎症。
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