HKOCl-4: a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid in living cells and tissues,Organic Chemistry Frontiers

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HKOCl-4: a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid in living cells and tissues
Organic Chemistry Frontiers ( IF 4.6 ) Pub Date : 2020-03-10 , DOI: 10.1039/d0qo00081g
Xiaoyu Bai _{1,

2,

3,

4} , Bowei Yang _{1,

2,

3,

4} , Hansen Chen _{2,

3,

4,

5} , Jiangang Shen _{2,

3,

4,

5} , Dan Yang _{1,

2,

3,

4}

Affiliation

In order to understand the production and functions of HOCl in biological systems, a sensitive and selective detection method is urgently needed, especially for HOCl visualization in living cells and tissues. Herein we report a highly sensitive and selective rhodol-based fluorescent probe HKOCl-4 for HOCl detection. HKOCl-4r and HKOCl-4m, which were designed for better cellular uptake and mitochondria-targeting, respectively, have been successfully applied in detecting endogenous HOCl in macrophages. Furthermore, HOCl production in ischemic rat brain tissues has been visualized with HKOCl-4r in a middle cerebral artery occlusion (MCAO) model. These probes would serve as powerful imaging tools for delving into the roles of HOCl in health and disease.

中文翻译：

HKOCl-4：一种基于Rhodol的黄色荧光探针，用于检测活细胞和组织中的次氯酸

为了理解HOCl在生物系统中的产生和功能，迫切需要灵敏且选择性的检测方法，尤其是对于活细胞和组织中HOCl的可视化。在本文中，我们报道了用于HOC1检测的高度灵敏且选择性的基于Rhodol的荧光探针HKOC1-4。分别设计用于更好的细胞摄取和线粒体靶向的HKOCl-4r和HKOCl-4m已成功应用于检测巨噬细胞中的内源性HOCl。此外，使用HKOCl-4r可以观察到缺血性大鼠脑组织中的HOCl生成在大脑中动脉闭塞（MCAO）模型中。这些探针将用作研究HOC1在健康和疾病中的作用的强大成像工具。

更新日期：2020-04-24

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