当前位置: X-MOL 学术Org. Chem. Front. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
HKOCl-4: a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid in living cells and tissues
Organic Chemistry Frontiers ( IF 4.6 ) Pub Date : 2020-03-10 , DOI: 10.1039/d0qo00081g
Xiaoyu Bai 1, 2, 3, 4 , Bowei Yang 1, 2, 3, 4 , Hansen Chen 2, 3, 4, 5 , Jiangang Shen 2, 3, 4, 5 , Dan Yang 1, 2, 3, 4
Affiliation  

In order to understand the production and functions of HOCl in biological systems, a sensitive and selective detection method is urgently needed, especially for HOCl visualization in living cells and tissues. Herein we report a highly sensitive and selective rhodol-based fluorescent probe HKOCl-4 for HOCl detection. HKOCl-4r and HKOCl-4m, which were designed for better cellular uptake and mitochondria-targeting, respectively, have been successfully applied in detecting endogenous HOCl in macrophages. Furthermore, HOCl production in ischemic rat brain tissues has been visualized with HKOCl-4r in a middle cerebral artery occlusion (MCAO) model. These probes would serve as powerful imaging tools for delving into the roles of HOCl in health and disease.

中文翻译:

HKOCl-4:一种基于Rhodol的黄色荧光探针,用于检测活细胞和组织中的次氯酸

为了理解HOCl在生物系统中的产生和功能,迫切需要灵敏且选择性的检测方法,尤其是对于活细胞和组织中HOCl的可视化。在本文中,我们报道了用于HOC1检测的高度灵敏且选择性的基于Rhodol的荧光探针HKOC1-4。分别设计用于更好的细胞摄取和线粒体靶向的HKOCl-4rHKOCl-4m已成功应用于检测巨噬细胞中的内源性HOCl。此外,使用HKOCl-4r可以观察到缺血性大鼠脑组织中的HOCl生成在大脑中动脉闭塞(MCAO)模型中。这些探针将用作研究HOC1在健康和疾病中的作用的强大成像工具。
更新日期:2020-04-24
down
wechat
bug