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Purification and enrichment of specific chromatin loci.
Nature Methods ( IF 36.1 ) Pub Date : 2020-03-09 , DOI: 10.1038/s41592-020-0765-4 Mathilde Gauchier 1 , Guido van Mierlo 2 , Michiel Vermeulen 2 , Jérôme Déjardin 1
中文翻译：
纯化和富集特定的染色质基因座。
更新日期：2020-03-09
Nature Methods ( IF 36.1 ) Pub Date : 2020-03-09 , DOI: 10.1038/s41592-020-0765-4 Mathilde Gauchier 1 , Guido van Mierlo 2 , Michiel Vermeulen 2 , Jérôme Déjardin 1
Affiliation
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Understanding how chromatin is regulated is essential to fully grasp genome biology, and establishing the locus-specific protein composition is a major step toward this goal. Here we explain why the isolation and analysis of a specific chromatin segment are technically challenging, independently of the method. We then describe the published strategies and discuss their advantages and limitations. We conclude by discussing why significant technology developments are required to unambiguously describe the composition of small single loci.
中文翻译：
纯化和富集特定的染色质基因座。
了解染色质如何调节对于充分掌握基因组生物学至关重要，而建立基因座特异性蛋白组成是朝着这一目标迈出的重要一步。在这里，我们解释了为何独立于方法而不是特定染色质片段的分离和分析在技术上具有挑战性。然后，我们描述已发布的策略并讨论其优势和局限性。最后，我们讨论了为什么需要显着的技术发展来明确描述小的单个基因座的组成。
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