Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry.,Nature Methods

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Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry.
Nature Methods ( IF 36.1 ) Pub Date : 2020-03-09 , DOI: 10.1038/s41592-020-0770-7
Tobias P Wörner _{1,

2} , Joost Snijder _{1,

2} , Antonette Bennett ₃ , Mavis Agbandje-McKenna ₃ , Alexander A Makarov _{1,

4} , Albert J R Heck _{1,

2}

Affiliation

We demonstrate single-particle charge detection mass spectrometry on an Orbitrap for the analysis of megadalton biomolecular assemblies. We establish that the signal amplitudes of individual ions scale linearly with their charge, which can be used to resolve mixed ion populations, determine charge states and thus also determine the masses of individual ions. This enables the ultrasensitive analysis of heterogeneous protein assemblies including immunoglobulin oligomers, ribosomes, proteinaceous nanocontainers and genome-packed adeno-associated viruses.

中文翻译：

通过基于Orbitrap的单粒子电荷检测质谱法解析异构大分子组装体。

我们演示了Orbitrap上的单粒子电荷检测质谱仪，用于分析兆达尔顿生物分子组装体。我们建立了单个离子的信号幅度随其电荷线性变化的特性，可用于解析混合离子种群，确定电荷状态并从而确定单个离子的质量。这使得能够对包括免疫球蛋白寡聚物，核糖体，蛋白质纳米容器和基因组包装的腺相关病毒在内的异种蛋白质组件进行超灵敏分析。

更新日期：2020-03-09

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