Superior field performance of waxy corn engineered using CRISPR-Cas9.,Nature Biotechnology

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Superior field performance of waxy corn engineered using CRISPR-Cas9.
Nature Biotechnology ( IF 33.1 ) Pub Date : 2020-03-09 , DOI: 10.1038/s41587-020-0444-0
Huirong Gao ₁ , Mark J Gadlage ₁ , H Renee Lafitte _{1,

2} , Brian Lenderts ₁ , Meizhu Yang ₁ , Megan Schroder ₁ , Jeffry Farrell ₁ , Kay Snopek ₁ , Dave Peterson ₁ , Lanie Feigenbutz ₁ , Spencer Jones ₁ , Grace St Clair ₁ , Melissa Rahe ₁ , Nathalie Sanyour-Doyel ₁ , Chenna Peng ₁ , Lijuan Wang ₁ , Joshua K Young ₁ , Mary Beatty ₁ , Brian Dahlke ₁ , Jan Hazebroek ₁ , Thomas W Greene ₁ , A Mark Cigan _{1,

3} , N Doane Chilcoat ₁ , R Bob Meeley ₁

Affiliation

We created waxy corn hybrids by CRISPR-Cas9 editing of a waxy allele in 12 elite inbred maize lines, a process that was more than a year faster than conventional trait introgression using backcrossing and marker-assisted selection. Field trials at 25 locations showed that CRISPR-waxy hybrids were agronomically superior to introgressed hybrids, producing on average 5.5 bushels per acre higher yield.

中文翻译：

使用CRISPR-Cas9设计的糯玉米具有卓越的田间性能。

我们通过CRISPR-Cas9编辑12个优良自交系玉米的蜡质等位基因，创建了蜡质玉米杂种，该过程比使用回交和标记辅助选择的常规性状基因渗入要快一年以上。在25个地点进行的田间试验表明，CRISPR蜡质杂种在农艺学上优于渗入杂种，每英亩平均产量提高5.5蒲式耳。

更新日期：2020-04-24

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