Key message

BoFL, a candidate gene conferring the feathered-leaved trait in ornamental kale, is located in a 374.532-kb region on chromosome C9.

Abstract

Leaf shape is an important economic trait in ornamental kale (Brassica oleracea var. acephala). Identifying the genes that influence leaf shape would provide insight into the mechanism underlying leaf development. In this study, we constructed F₁, F₂, BC₁P₁, BC₁P₂, and F_2:3 populations from a cross between a feathered-leaved inbred line, F0819, and a smooth-leaved inbred line, S0835, of ornamental kale. Genetic analysis showed that the feathered-leaved trait was controlled by a semi-dominant gene, BoFL. Using bulked segregant analysis sequencing, we mapped the BoFL gene to a 4.17-Mb interval on chromosome C9. Next, we narrowed down the candidate region to 374.532-kb by fine-scale mapping between the two flanking markers INDEL940 and INDEL5443. We identified 38 genes in the candidate region, among which only Bo9g184610 showed significant variation in expression level between the two parental lines. Sequencing of the gene in the parental lines identified three single-nucleotide polymorphism (SNP) differences, containing two nonsynonymous and one synonymous SNPs, which resulted in coding variations of an aspartate and a phenylalanine residue in F0819, but an alanine and a leucine residue in S0835. A cleaved amplified polymorphic sequence (CAPS) marker, CAPS4610, corresponding to the first nonsynonymous SNP co-segregated with the leaf shape trait. We thus speculated that the gene conferring the feathered-leaved trait is BoALG10, a homolog of ALG10, which encodes an alpha-1,2-glucosyltransferase in Arabidopsis thaliana. This work will be useful for understanding the mechanism of leaf development and provides important information for the breeding of kale with novel leaf shapes.

中文翻译：

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羽衣甘蓝叶形基因BoFL的精细定位与鉴定

关键信息

BoFL是一种赋予观赏羽衣甘蓝羽状叶性状的候选基因，位于C9染色体的374.532-kb区域。

抽象

叶形是观赏羽衣甘蓝（甘蓝型油菜变种acephala）的重要经济性状。鉴定影响叶片形状的基因将提供对叶片发育潜在机制的深入了解。在这项研究中，我们从羽毛有叶的近交系F0819和光滑有叶的近交系S0835的杂交中构建了F ₁，F ₂，BC ₁ P ₁，BC ₁ P ₂和F _2：3种群。 ，观赏羽衣甘蓝。遗传分析表明，羽状性状受半显性基因BoFL的控制。使用大量分离剂分析测序，我们绘制了BoFL基因在C9染色体上的间隔为4.17-Mb。接下来，我们通过两个侧翼标记INDEL940和INDEL5443之间的精细映射将候选区域缩小到374.532-kb。我们在候选区域中鉴定了38个基因，其中只有Bo9g184610显示出两个亲本系之间表达水平的显着变化。亲本系中基因的测序确定了三个单核苷酸多态性（SNP）差异，其中包含两个非同义和一个同义SNP，导致F0819中天冬氨酸和苯丙氨酸残基的编码变异，但在F0819中存在丙氨酸和亮氨酸残基。 S0835。切割的扩增多态序列（CAPS）标记CAPS4610，对应于与叶形性状共分离的第一个非同义SNP。因此，我们推测，基因，赋予顺桨叶性状是BoALG10，的同源物ALG10，其在编码的α-1,2-葡糖基转移酶的拟南芥。这项工作将有助于理解叶片发育的机制，并为具有新颖叶片形状的羽衣甘蓝育种提供重要信息。