Abstract In this study, a new dual-signal electrochemical sensor using a glassy carbon electrode (GCE) modified with molecularly imprinted polyaniline/polythionine/gold nanoparticles@zeolitic imidazolate framework-67 composite (MIP/pTH/Au@ZIF-67) is fabricated for the quantitative analysis of tyrosine (Tyr). Au@ZIF-67 is used as a matrix to improve the surface area and electron-transfer ability of the sensor. The pTH as a built-in probe provides a signal-off response, meanwhile the oxide current of Tyr acts as a signal-on response. The MIP is prepared in the presence of aniline monomer and Tyr template by electro-polymerization for Tyr specific recognition. Under the optimized conditions, the sensor demonstrates a linear range from 1 × 10−8 M to 4 × 10−6 M for Tyr determination and a limit of detection (LOD) of 7.9 × 10−10 M (S/N = 3). Furthermore, the sensor exhibits excellent selectivity, superior stability and good reproducibility toward Tyr. More importantly, the sensor is successfully employed to detect Tyr in human serum with satisfactory recoveries.

中文翻译：

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Au纳米颗粒@金属有机骨架/聚硫氨酸负载分子印迹聚合物传感器：酪氨酸的制备、表征和电化学检测

摘要 本研究采用分子印迹聚苯胺/聚硫氨酸/金纳米颗粒@沸石咪唑酯骨架-67复合材料（MIP/pTH/Au@ZIF-67）修饰的玻碳电极（GCE）制备了一种新型双信号电化学传感器。用于酪氨酸 (Tyr) 的定量分析。Au@ZIF-67 用作基质以提高传感器的表面积和电子转移能力。作为内置探针的 pTH 提供信号关闭响应，同时 Tyr 的氧化物电流作为信号开启响应。MIP 在苯胺单体和 Tyr 模板的存在下通过电聚合制备 Tyr 特异性识别。在优化条件下，传感器显示出从 1 × 10−8 M 到 4 × 10−6 M 的 Tyr 测定线性范围和 7.9 × 10−10 M (S/N = 3) 的检测限 (LOD) . 此外，该传感器对 Tyr 表现出出色的选择性、出色的稳定性和良好的重现性。更重要的是，该传感器成功地用于检测人血清中的 Tyr，回收率令人满意。