Abstract Our previous work demonstrated the presence of compounds with hypocholesterolemic capacity in olive seeds. These compounds were extracted using CO2-expanded ethyl acetate and identified as tyrosol, hydroxytyrosol, and β-sitosterol using GC–MS. This work describes the extraction of these compounds from different olive seeds using pressurized ethyl acetate. Their solubility in ethyl acetate at temperatures ranging from 40–200 °C was theoretically predicted by Hansen solubility parameters. The content of these compounds was estimated by GC–MS, as well as, the reduction of the micellar cholesterol solubility (RMCS) capacity of extracts enabling to establish the optimum extraction temperature at 100 °C. A GC–MS method was developed and validated in terms of its analytical characteristics for a sensitive determination and quantification of tyrosol, hydroxytyrosol, and β-sitosterol in different olive seeds. Within varieties, Manzanilla seeds presented the highest concentration of tyrosol, hydroxytyrosol, and the lowest concentration of β-sitosterol, as well as the highest RMCS capacity.

中文翻译：

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采用加压液体萃取和气相色谱-质谱法从油橄榄种子中回收和测定降胆固醇化合物

摘要我们之前的工作证明了橄榄种子中存在具有降胆固醇能力的化合物。这些化合物使用 CO2 膨胀的乙酸乙酯提取，并使用 GC-MS 鉴定为酪醇、羟基酪醇和 β-谷甾醇。这项工作描述了使用加压乙酸乙酯从不同橄榄种子中提取这些化合物。Hansen 溶解度参数理论上预测了它们在 40-200°C 温度范围内在乙酸乙酯中的溶解度。这些化合物的含量通过 GC-MS 以及提取物胶束胆固醇溶解度 (RMCS) 能力的降低来估计，从而能够在 100 °C 下建立最佳提取温度。开发并验证了 GC-MS 方法的分析特性，用于灵敏测定和定量不同橄榄种子中的酪醇、羟基酪醇和 β-谷甾醇。在品种中，Manzanilla 种子呈现出最高浓度的酪醇、羟基酪醇和最低浓度的 β-谷甾醇，以及最高的 RMCS 容量。