Abstract Hydrazine (N2H4) has been widely used in many areas of the chemical industry, but it can cause potential harm to human health and environmental pollution. Herein, a novel fluorescent probe (L) benefit from smart cyclization mechanism has been constructed for ratio detection of hydrazine. Due to the destruction of the conjugate system by cyclic reaction, the intramolecular charge transfer (ICT) process was blocked, and a large hypsochromic shift could be observed both in the absorption and fluorescence spectra with excellent ratio response. The sensing mechanism was also studied by NMR spectra and DFT calculation. This ratiometric probe was highly selective and sensitive for detecting hydrazine with a low detection limit of 0.23 μM. Moreover, that probe was also effective in monitoring hydrazine in live cells.

中文翻译：

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基于智能反应的联氨比例检测荧光探针及其在活细胞中的应用

摘要 肼（N2H4）已广泛应用于化学工业的许多领域，但它可能对人体健康和环境造成潜在危害。在此，构建了一种受益于智能环化机制的新型荧光探针 (L) 用于肼的比率检测。由于循环反应破坏了共轭体系，分子内电荷转移（ICT）过程被阻断，在吸收光谱和荧光光谱中都可以观察到较大的低色移，具有优异的比响应。还通过核磁共振谱和 DFT 计算研究了传感机制。这种比率探针对检测肼具有高度选择性和敏感性，检测限低至 0.23 μM。此外，该探针还可有效监测活细胞中的肼。