We previously demonstrated that the cockroach α-bungarotoxin-sensitive nicotinic acetylcholine receptors, nAChR1 and nAChR2 subtypes, are differently sensitive to intracellular calcium pathways. Here, using whole cell patch-clamp recordings, we studied the effects of the diacylglycerol (DAG) analogue 1,2-dioctanoyl-sn-glycerol (DiC8) on nicotine- and clothianidin-evoked currents under an α-bungarotoxin treatment. Our results demonstrated that DiC8 reduced nicotine and clothianidin evoked currents. 10 μM DiC8 suppressed the increase in nicotine-induced currents which was brought about by application of 5 mM caffeine or 9 mM Ca2+, whereas DiC8 did not affect the decrease in nicotine-induced currents induced by BAPTA. Similarly, bath application of caffeine or 9 mM Ca2+ did not change the clothianidin effects, and the amplitude of clothianidin-induced currents was not affected. However, co-application of both 10 μM DiC8 with 9 mM Ca2+, caffeine or BAPTA reduced clothianidin current amplitudes. We conclude that nicotine and clothianidin differently modulate nAChR1 and nAChR2 subtypes under DiC8 treatment, and that nicotine activates nAChR1, whereas clothianidin activates both nAChR1 and nAChR2 subtypes.

中文翻译：

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DAG类似物1,2-二辛酰基-sn-甘油（DiC8）通过在蟑螂神经分泌细胞上表达的对α-真菌毒素不敏感的烟碱乙酰胆碱受体对尼古丁和可尼丁引起的电流的影响。

我们以前证明了蟑螂α-真菌毒素敏感的烟碱型乙酰胆碱受体，nAChR1和nAChR2亚型​​，对细胞内钙途径的敏感性不同。在这里，我们使用全细胞膜片钳记录，研究了在α-真菌毒素处理下，二酰基甘油（DAG）类似物1,2-二辛酰基-sn-甘油（DiC8）对尼古丁和可尼丁引起的电流的影响。我们的结果表明，DiC8降低了尼古丁和可尼丁引起的电流。10μMDiC8抑制了因应用5 mM咖啡因或9 mM Ca2 +而引起的尼古丁诱导电流的增加，而DiC8则不影响BAPTA诱导的尼古丁诱导电流的减少。同样，在咖啡厅中浸泡咖啡因或9 mM Ca2 +不会改变可比尼丁的作用，可比尼丁诱导的电流幅度不受影响。但是，将10μMDiC8与9 mM Ca2 +，咖啡因或BAPTA并用会降低可比尼丁电流幅度。我们得出的结论是，在DiC8处理下，尼古丁和可比尼丁会不同地调节nAChR1和nAChR2亚型​​，尼古丁会激活nAChR1，而可比尼丁会同时激活nAChR1和nAChR2亚型​​。