Abstract Resource and time consuming difficult procedures involving the preparation of antigen surface functionalized nanoparticles mimicking viruses for optimum immunogenicity have been the challenges for their actual application. In this context, we developed stable polymeric particles functionalized with Ni2+-NTA via the combination of RAFT emulsion polymerization and polymerization-induced self-assembly (RAFT-PISA), and mixed with recombinant H1 tagged with hexa-histidine (His6-H1) for one day to prepare the influenza-mimetic polymeric particles. The RAFT-PISA based polymer particles exhibited good structural stability while the Ni2+-NTA-functionality provided a perfect docking site for the controlled orientation of His6-H1. The His6-H1 protein immobilization with Ni2+-NTA of the particle was studied with time. In addition, we investigated the immune response of the synthesized vaccine against influenza with varying the mixing time. We expect our system would open new avenues for preparation of influenza-mimetic vaccines like by mixing of a ready-made surface-functionalized particle and recombinant hemagglutinin.

中文翻译：

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用于病毒模拟流感疫苗的基于 RAFT/PISA 的 Ni-NTA 聚合物颗粒

摘要 涉及制备抗原表面功能化纳米颗粒以实现最佳免疫原性的抗原表面功能化纳米颗粒的制备过程既费时又费力，这一直是其实际应用的挑战。在此背景下，我们通过 RAFT 乳液聚合和聚合诱导自组装 (RAFT-PISA) 的组合开发了用 Ni2+-NTA 功能化的稳定聚合物颗粒，并与用六组氨酸 (His6-H1) 标记的重组 H1 混合用于一天制备流感模拟聚合物颗粒。基于 RAFT-PISA 的聚合物颗粒表现出良好的结构稳定性，而 Ni2+-NTA 功能为 His6-H1 的受控取向提供了完美的对接位点。随着时间的推移，研究了用颗粒的 Ni2+-NTA 固定 His6-H1 蛋白质。此外，我们研究了不同混合时间的合成疫苗对流感的免疫反应。我们预计我们的系统将为制备流感模拟疫苗开辟新途径，例如通过混合现成的表面功能化颗粒和重组血凝素。