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Phenolic content and antioxidant activity of flixweed (Descurainia sophia) seeds extracts: Ranking extraction systems based on fuzzy logic method
Sustainable Chemistry and Pharmacy ( IF 5.5 ) Pub Date : 2020-03-09 , DOI: 10.1016/j.scp.2020.100245
Bahar Shahidi , Akram Sharifi , Leila Roozbeh Nasiraie , Mehrdad Niakousari , Mohammad Ahmadi

Flixweed (Descurainia sophia) seeds (FS) is a rich source of polyphenols. This study aimed to investigate the effects of various solvents (water, aqueous-methanol and aqueous-ethanol) and techniques (maceration (M), ultrasound-assisted extraction (UAE), ohmic-assisted extraction (OAE), and decoction – infusion (DI)) on extraction yield, pH, total phenolic contents (TPC), and antioxidant activity (DPPH radical scavenging activity (DRSA)) of flixweed seeds powder (FSP). Extraction systems were ranked by fuzzy-logic (inputs: extraction yield and time, TPC and DRSA). Water as a solvent in extraction process had the maximum yield, TPC and DRSA 6.49%; 141.598 mg GAE/100 mL and 72.91%, respectively. The difference between water and aqueous-ethanol was significant (p < 0.05). Values of pH of aqueous-methanol extract was significantly higher than other solvents. OAE and UAE achieved the highest (10.1%) and lowest (2.3%) extraction yields, respectively. Low temperature maceration showed the highest TPC and DRSA. Fuzzy-logic ranking based on expert's knowledge (30 experts) predicted the OAE with water as solvent to be score the highest rank in terms of overall efficiency. The overall efficiency is determined based on yield, extraction duration, antioxidant activity, and TPC. However, if the ranking are to be based on isolation of bioactive materials at a reasonably short time, the UAE may be the preferred choice. The HPLC analysis of OAE water extract indicates the presence of several phenolic compounds such as caffeic acid, p-coumaric acid, rutin, quercetin, and kaempferol.



中文翻译:

酚含量和flixweed(抗氧化活性播娘蒿)种子提取物:基于模糊逻辑的方法排名提取系统

lix草(Descurainia sophia)种子(FS)是多酚的丰富来源。这项研究旨在研究各种溶剂(水,甲醇水溶液和乙醇水溶液)和技术(浸渍(M),超声辅助萃取(UAE),欧姆辅助萃取(OAE)以及煎煮-输注( DI))对亚麻草粉(FSP)的提取率,pH,总酚含量(TPC)和抗氧化活性(DPPH自由基清除活性(DRSA))的影响。提取系统通过模糊逻辑进行排序(输入:提取产量和时间,TPC和DRSA)。萃取过程中以水为溶剂的收率最高,TPC和DRSA为6.49%。分别为141.598 mg GAE / 100 mL和72.91%。水和乙醇水溶液之间的差异显着(p <0.05)。甲醇水溶液的pH值明显高于其他溶剂。OAE和阿联酋分别获得了最高的提取率(10.1%)和最低的(2.3%)。低温浸渍显示最高的TPC和DRSA。基于专家知识的模糊逻辑排名(有30位专家)预测,以水为溶剂的OAE在总体效率方面得分最高。总效率取决于产量,提取时间,抗氧化活性和TPC。但是,如果排名是基于在相当短的时间内分离出生物活性物质而得出的,则阿联酋可能是首选。OAE水提取物的HPLC分析表明存在几种酚类化合物,例如咖啡酸,对香豆酸,芦丁,槲皮素和山ka酚。

更新日期:2020-03-09
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