Tomato (Lycopersicon esculentum Mill [Solanum lycopersicum L.].) is an important food material and cash crop, as well as a model plant for genetic evolution and molecular biology research. However, as a cold-sensitive crop originating from the tropics, the growth and development of tomato is often affected by low temperature stress. Therefore, how processing tomatoes resist this type of stress has important theoretical and practical significance. In this study, the LeCOLD1 gene was cloned from processing tomato. Subcellular localization analysis showed that LeCOLD1 was located in the plasma membrane. Real-time quantitative PCR analysis showed that LeCOLD1 was highly expressed in roots. Drought, salt and low temperatures induced the expression of COLD1. Overexpression and RNA interference vectors of LeCOLD1 were constructed and were transformed into tomato by the Agrobacterium-mediated method, and then obtaining transgenic tomato plants. It was found that LeCOLD1 increased the height of processing tomato plants and increased the length of their roots. In addition, overexpression of LeCOLD1 significantly improved the cold resistance of the plants. Overexpressing LeCOLD1 in tomato plants reduced the damage to the cell membrane, accumulation of ROS and photoinhibition of PSII, and maintained the high activity of antioxidant enzymes and the content of osmotic regulators. Further analysis revealed that during low temperature stress, the cells maintained high levels of antioxidant enzyme activity by regulating the transcription of the genes encoding these enzymes. The results show that overexpressing LeCOLD1 in tomato increases the plants' resistance to low temperatures, and that reducing LeCOLD1 expression makes the plants more sensitive to low temperatures.

中文翻译：

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耐冷性差异1蛋白赋予加工番茄耐冷性。

番茄（Lycopersicon esculentum Mill [Solanum lycopersicum L.]。）是重要的粮食原料和经济作物，也是用于遗传进化和分子生物学研究的模型植物。但是，作为来自热带地区的对冷敏感的作物，番茄的生长发育常常受到低温胁迫的影响。因此，加工番茄如何抵抗这种压力具有重要的理论和实践意义。在这项研究中，从加工番茄中克隆了LeCOLD1基因。亚细胞定位分析表明LeCOLD1位于质膜中。实时定量PCR分析表明，LeCOLD1在根中高表达。干旱，盐分和低温诱导了COLD1的表达。构建了LeCOLD1的过表达和RNA干扰载体，并通过农杆菌介导的方法将其转化为番茄，获得了转基因番茄植株。发现LeCOLD1增加了加工番茄植物的高度并增加了其根的长度。另外，LeCOLD1的过表达显着改善了植物的抗寒性。番茄植株中过表达的LeCOLD1减少了对细胞膜的破坏，ROS的积累和PSII的光抑制，并维持了抗氧化酶的高活性和渗透调节剂的含量。进一步的分析表明，在低温胁迫下，细胞通过调节编码这些酶的基因的转录来维持高水平的抗氧化酶活性。