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Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity.
Immunity ( IF 25.5 ) Pub Date : 2020-03-10 , DOI: 10.1016/j.immuni.2020.02.001
Thomas M Fenton 1 , Peter B Jørgensen 1 , Kristoffer Niss 2 , Samuel J S Rubin 3 , Urs M Mörbe 4 , Lene B Riis 5 , Clément Da Silva 4 , Adam Plumb 6 , Julien Vandamme 1 , Henrik L Jakobsen 7 , Søren Brunak 2 , Aida Habtezion 3 , Ole H Nielsen 8 , Bengt Johansson-Lindbom 9 , William W Agace 10
Affiliation  

The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.

中文翻译:


人类肠道相关淋巴组织的免疫分析确定了孤立的淋巴滤泡在区域特异性免疫启动中的作用。



肠道包含体内一些最多样化和最复杂的免疫区室。在这里,我们描述了一种分离人肠道相关淋巴组织 (GALT) 的方法,该方法可以对粘膜下和粘膜分离淋巴滤泡(分别为 SM-ILF 和 M-ILF)以及无 GALT 的适应性免疫系统进行前所未有的分析。肠固有层(LP)。 SM-ILF 和 M-ILF 在肠道长度上显示出不同的分布模式,通过 MAdCAM-1+ 高内皮微静脉和传出淋巴管与体循环相关,并且具有与免疫诱导位点一致的免疫特征。 IgA 测序分析表明,人类 ILF 是肠道适应性免疫反应以解剖学限制方式启动的位点。我们的研究结果将 ILF 定位为人类肠道区域免疫的关键诱导中心,所提出的方法将允许未来对这些区室的健康和疾病进行评估。
更新日期:2020-03-10
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