Quantifying the microRNA (miRNA) level and manipulating them in complex samples, such as serum, is of intense interest because miRNAs are important diagnostic markers. Here, we demonstrate an optical microfiber integrating of untrasensitive detection function and local photothermal therapy potential. A nanointerface consisting of GO supported Cu_2-xS nanoplates presented the localized surface plasmon resonance (LSPR) tuned to be consistent with the operation wavelength of the microfiber transducer. It enhanced the surface energy density of evanescent field, on which the miRNA sensing and therapy occurred. With evanescent field enhancement by the plasmonic nanointerface, the sensor exhibits an ultrahigh sensitivity for detecting microRNA at concentrations ranging from 0.1 aM to 10 pM. It is also capable of differentiating one-base mismatches of miRNA at ultralow concentrations (as low as 10 aM) in serum. The photothermal effect of nanointerface simultaneously endows the sensor with the potential for localized photothermal therapy. This work presents a possible approach for the in-situ integration of diagnosis and treatment in early stage.

由于miRNA是重要的诊断标记，因此量化microRNA（miRNA）的水平并在复杂的样品（如血清）中进行操作非常受关注。在这里，我们展示了不敏感检测功能和局部光热疗法潜力的光学微纤维。由GO支持的Cu _2-x组成的纳米界面S纳米板呈现的局部表面等离子体共振（LSPR）已调整为与微纤维换能器的工作波长一致。它增强了e逝场的表面能密度，在RNA逝场上发生了miRNA感应和治疗。随着等离子体纳米界面的消逝场增强，该传感器在检测浓度范围从0.1 aM到10 pM的microRNA时表现出超高的灵敏度。它还能够区分血清中超低浓度（低至10 aM）的miRNA的一碱基错配。纳米界面的光热效应同时赋予传感器局部光热疗法的潜力。这项工作为早期诊断和治疗的原位整合提供了一种可能的方法。