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Correction to Copper Influences the Antibacterial Outcomes of a β-Lactamase-Activated Prochelator against Drug-Resistant Bacteria.
ACS Infectious Diseases ( IF 4.0 ) Pub Date : 2020-03-10 , DOI: 10.1021/acsinfecdis.0c00082
Jacqueline M. Zaengle-Barone , Abigail C. Jackson , David M. Besse , Bradford Becken , Mehreen Arshad , Patrick C. Seed , Katherine J. Franz

In the Materials section, compound 1 should be 4-methoxybenzyl (6R,7R)-3-(chloromethyl)-8-oxo-7-(2-phenylacetamido)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate. Discrepancies uncovered in follow-up studies lead to the re-evaluation of NDM-1-containing strains. The conclusions of the original manuscript remain unchanged, but the following figures and tables should be updated with values for the correct NDM-1-containing strains. Figure 2: The concentration of PcephPT after incubation with E. coli MG1655 expressing NDM-1 was 45 ± 3 μM after 1 h and 4 ± 6 μM after 20 h. Table 2: PcephPT did not fully inhibit the growth of either NDM-1 strain; values of 17.5 μM for PcephPT in MG1655 are more accurately described as MIC90 (compound only) and MIC60 (+Cu), while analogous entries for UTI89 NDM-1 reflect MIC60 and MIC70, respectively. PT in UTI89 had an MIC of 17.5 μM. Table 3: PcephPT inhibited the NDM-1 copAΔ strain with an MIC60 of 8.8 μM (compound alone); MIC of PcephPT + Cu was 17.5 μM, with an MIC80 of 1.1 μM. Figure 4: CFU/mL for the MG1655 NDM-1 strain was >12 500 when treated with PT and 0–500 when treated with PT + Cu. Figure S9: CFU/mL for the UTI89 NDM-1 strain were 4500–8500 when treated with PT and 0–500 when treated with PT + Cu. Cells treated with PcephPT ± Cu were not spotted due to high MICs. 0 h CFU/mL values: MG1655 NDM-1 = 1 100 000; UTI89 NDM-1 = 940 000. This article has not yet been cited by other publications.

中文翻译:

铜的校正影响β-内酰胺酶激活的Prochelator对抗药性细菌的抗菌效果。

在“材料”部分中,化合物1应为4-甲氧基苄基(6 R,7 R)-3-(氯甲基)-8-氧代-7-(2-苯基乙酰胺基)-5-硫杂-1-氮杂双环[4.2.0]辛二烯-2-羧酸酯。在后续研究中发现的差异导致对包含NDM-1的菌株的重新评估。原始手稿的结论保持不变,但以下图和表应使用正确的含NDM-1菌株的值进行更新。图2:与表达NDM-1的大肠杆菌MG1655一起孵育后,PcephPT的浓度在1小时后为45±3μM,在20小时后为4± 6μM 。表2:PcephPT不能完全抑制任一NDM-1菌株的生长。MG1655中PcephPT的17.5μM值更准确地描述为MIC 90(仅化合物)和MIC 60(+ Cu),而UTI89 NDM-1的类似条目分别反映了MIC 60和MIC 70。UTI89中的PT的MIC为17.5μM。表3:PcephPT以8.8μM的MIC 60(仅化合物)抑制NDM-1copAΔ菌株;PcephPT + Cu的MIC为17.5μM,MIC 80为1.1μM 。图4:使用PT处理的MG1655 NDM-1菌株的CFU / mL> 12 500,使用PT + Cu处理的CFU / mL为0–500。图S9:UTI89 NDM-1菌株经PT处理后的CFU / mL为4500–8500,而经PT + Cu处理时为0–500。由于高MIC,未发现用PcephPT±Cu处理的细胞。0 h CFU / mL值:MG1655 NDM-1 = 1 100 000; UTI89 NDM-1 = 940000。其他出版物尚未引用此文章。
更新日期:2020-04-23
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