Phytochemical analysis of a methanol-dichloromethane (1:1) extract of the aerial parts of Tephrosialinearis led to the isolation of 18 compounds. Seven of these, namely, lineaflavones A-D (1-4), 6-methoxygeraldone (5), 8″-acetylobovatin (6), and 5-hydroxy-7-methoxysaniculamin A (7) are new compounds. The compounds were characterized based on their NMR and HRMSn data. The anti-inflammatory effects of the crude extract and isolated compounds were evaluated by measuring the levels of interleukins (IL-1β, IL-2, and IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs). The crude extract inhibited the release of all cytokines except IL-1β, which slightly increased in comparison to the LPS control. All the tested compounds suppressed the production of IL-2, GM-CSF, and TNF-α. Whereas compounds 1, 2, 4-8, 10-15, 17, and 18 decreased production of IL-6, compounds 1, 2, 4, 7, 10, 13-15, and 17 inhibited the release of IL-1β. It is worth noting that most of the compounds tested showed a superior reduction in cytokines release compared to the reference drug ibuprofen.

中文翻译：

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抗炎黄酮和黄酮类植物。

线虫地上部分的甲醇-二氯甲烷（1：1）提取物的植物化学分析导致​​分离出18种化合物。这些中的七个，即利奈黄酮AD（1-4），6-甲氧基杰拉酮（5），8''-乙酰基卵磷脂（6）和5-羟基-7-甲氧基Saniculamin A（7）是新化合物。根据其NMR和HRMSn数据对化合物进行表征。通过测量白介素（IL-1β，IL-2和IL-6），粒细胞巨噬细胞集落刺激因子（GM-CSF）和肿瘤的水平来评估粗提物和分离的化合物的抗炎作用脂多糖（LPS）刺激的外周血单核细胞（PBMC）中的坏死因子-α（TNF-α）。粗提取物抑制除IL-1β以外的所有细胞因子的释放，与ILS对照相比，IL-1β略有增加。所有测试的化合物均可抑制IL-2，GM-CSF和TNF-α的产生。化合物1、2、4、8、10、15、17和18降低了IL-6的产生，而化合物1，2、4、7、10、13、15和17抑制了IL-1β的释放。值得注意的是，与参考药物布洛芬相比，大多数测试化合物均显示出细胞因子释放的优异降低。