A novel CCCH‐type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice,Journal of Integrative Plant Biology

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A novel CCCH‐type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-03-09 , DOI: 10.1111/jipb.12924
Bin Wang _{1,

2,

3,

4} , Ruiqiu Fang _{1,

2,

5} , Faming Chen _{1,

4} , Jingluan Han _{1,

2,

3,

4} , Yao-Guang Liu _{1,

2,

4} , Letian Chen _{1,

2,

3,

4} , Qinlong Zhu _{1,

2,

4}

Affiliation

Male sterility is a prerequisite for hybrid seed production. The phytohormone gibberellin (GA) is involved in regulating male reproductive development, but the mechanism underlying GA homeostasis in anther development remains less understood. Here, we report the isolation and characterization of a new positive regulator of GA homeostasis, swollen anther wall 1 (SAW1), for anther development in rice (Oryza sativa L.). Rice plants carrying the recessive mutant allele saw1 produces abnormal anthers with swollen anther wall and aborted pollen. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRIPSR‐associated protein 9‐mediated knockout of SAW1 in rice generated similar male sterile plants. SAW1 encodes a novel nucleus‐localizing CCCH‐tandem zinc finger protein, and this protein could directly bind to the promoter region of the GA synthesis gene OsGA20ox3 to induce its anther‐specific expression. In the saw1 anther, the significantly decreased OsGA20ox3 expression resulted in lower bioactive GA content, which in turn caused the lower expression of the GA‐inducible anther‐regulator gene OsGAMYB. Thus, our results disclose the mechanism of the SAW1‐GA20ox3‐GAMYB pathway in controlling rice anther development, and provide a new target gene for the rapid generation of male sterile lines by genome editing for hybrid breeding.

中文翻译：

新型CCCH型锌指蛋白SAW1激活OsGA20ox3，以调节赤霉素稳态和水稻花药发育

雄性不育是杂交种子生产的前提。植物激素赤霉素（GA）参与调节雄性生殖发育，但在花药发育中GA稳态的潜在机制仍知之甚少。在这里，我们报告了GA稳态的新正调节剂，膨胀的花药壁1（SAW1），用于水稻（Oryza sativa L.）花药发育的分离和鉴定。带有隐性突变等位基因saw1的水稻植株产生异常的花药，其花药壁肿胀且花粉流产。水稻中成簇的规则间隔的短回文重复序列（CRISPR）/ CRIPSR相关蛋白9介导的SAW1的敲除产生了类似的雄性不育植物。SAW1编码一种新颖的核定位CCCH串联锌指蛋白，该蛋白可以直接与GA合成基因OsGA20ox3的启动子区域结合，以诱导其花药特异性表达。在锯木花药中，OsGA20ox3表达的显着降低导致生物活性GA含量降低，进而导致GA诱导的花药调节基因OsGAMYB的表达降低。因此，我们的结果揭示了SAW1 - GA20ox3-GAMYB途径在控制水稻花药发育中的机制，并为通过杂交育种的基因组编辑快速产生雄性不育系提供了新的靶基因。

更新日期：2020-03-09

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