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Botanical origin authentication of dietary supplements by DNA‐based approaches
Comprehensive Reviews in Food Science and Food Safety ( IF 12.0 ) Pub Date : 2020-03-06 , DOI: 10.1111/1541-4337.12551
Liliana Grazina 1 , Joana S Amaral 2 , Isabel Mafra 1
Affiliation  

Herbal products, such as dietary supplements, have become a subject of increasing global importance for their health benefits and economic considerations. However, they have also been targets of adulteration practices, being the accurate identification of botanicals in herbal products of utmost importance to protect the health and expectations of consumers. Particularly, in the case of dietary supplements, which can have different types of formulations, the identification of plant material used in their production is often a research challenge. DNA‐based techniques have played a crucial role on the development of a wide range of tools for the authentication of herbal products. Therefore, this review intends to describe their main progresses, critically discussing their advantages and drawbacks when applied to authenticate herbal products, focusing on dietary supplements. DNA barcoding is particularly emphasized because it has provided the highest number of applications, followed by the advances on high‐resolution melting analysis combined with DNA barcodes. A special emphasis is also given to the promising approaches relying on DNA metabarcoding and isothermal amplification.

中文翻译:

通过基于DNA的方法对膳食补充剂进行植物来源认证

诸如膳食补充剂的草药产品由于其健康益处和经济考虑已成为全球日益重要的主题。但是,它们也一直是掺假做法的目标,这是对草药产品中的植物药进行准确鉴定,这对于保护消费者的健康和期望至关重要。特别地,对于可以具有不同类型的制剂的膳食补充剂,鉴定用于其生产中的植物材料通常是研究挑战。基于DNA的技术在开发多种用于鉴定草药产品的工具方面发挥了至关重要的作用。因此,本综述旨在描述其主要进展,并批判性地讨论其在用于认证草药产品时的优缺点,专注于膳食补充剂。特别强调DNA条形码,因为它提供了最多的应用程序,其次是高分辨率熔解分析与DNA条形码的结合。还特别强调了依赖DNA元条形码和等温扩增的有前途的方法。
更新日期:2020-03-06
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