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An electrochemical sensor based on enzyme-free recycling amplification for sensitive and specific detection of miRNAs from cancer cells.
Analyst ( IF 3.6 ) Pub Date : 2020-03-30 , DOI: 10.1039/d0an00275e Lili Jiang 1 , Yuling Yang , Yuhong Lin , Ziyi Chen , Chao Xing , Chunhua Lu , Huanghao Yang , Shusheng Zhang
Analyst ( IF 3.6 ) Pub Date : 2020-03-30 , DOI: 10.1039/d0an00275e Lili Jiang 1 , Yuling Yang , Yuhong Lin , Ziyi Chen , Chao Xing , Chunhua Lu , Huanghao Yang , Shusheng Zhang
Affiliation
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MicroRNAs (miRNAs) are desirable targets for the diagnosis, prognosis and treatment of diverse human diseases. In this study, we developed a universal and enzyme-free signal amplification approach for the quick, sensitive and specific electrochemical detection of diverse miRNAs from tumor cells using a catalyzed hairpin assembly (CHA) and the DNA three-way junction (DNA TWJ). The target miRNA, which was formed as an initiator through specific recognition, periodically triggered the assembly of two hairpins into a duplex via CHA. Subsequently, these duplexes induced the catalytic assembly of the DNA three-way junction and the release of the methylene blue-labeled DNA strand S (S-MB). Thereafter, the S-MB was captured using the electrode through hybridization with the capture probe to generate a notable current response. The proposed biosensor could be switched in response to different miRNA targets by changing the specific sequence of H0. These findings indicate its ability to distinguish single base mismatch miRNAs and analyze miRNAs extracted from cells. Therefore, the universal and enzyme-free signal amplified electrochemical method described herein can be potentially useful in diverse miRNA-related clinical analysis and disease diagnosis.
中文翻译：
基于无酶回收扩增的电化学传感器，用于敏感和特异性地检测癌细胞中的miRNA。
MicroRNA（miRNA）是多种人类疾病的诊断，预后和治疗的理想靶标。在这项研究中，我们开发了一种通用且无酶的信号放大方法，用于使用催化发夹装配（CHA）和DNA三向连接（DNA TWJ）从肿瘤细胞中快速，灵敏和特异地电化学检测多种miRNA。通过特异性识别形成启动子的靶标miRNA定期触发两个发夹通过CHA组装成双链体。随后，这些双链体诱导了DNA三向连接的催化组装，并释放了亚甲基蓝标记的DNA链S（S-MB）。此后，通过与捕获探针杂交使用电极捕获S-MB，以产生显着的电流响应。通过改变H0的特定序列，可以响应于不同的miRNA靶标而切换提出的生物传感器。这些发现表明其区分单碱基错配miRNA和分析从细胞中提取的miRNA的能力。因此，本文所述的通用且无酶的信号放大电化学方法可潜在地用于与miRNA相关的各种临床分析和疾病诊断。
更新日期：2020-03-09
中文翻译：
基于无酶回收扩增的电化学传感器，用于敏感和特异性地检测癌细胞中的miRNA。
MicroRNA（miRNA）是多种人类疾病的诊断，预后和治疗的理想靶标。在这项研究中，我们开发了一种通用且无酶的信号放大方法，用于使用催化发夹装配（CHA）和DNA三向连接（DNA TWJ）从肿瘤细胞中快速，灵敏和特异地电化学检测多种miRNA。通过特异性识别形成启动子的靶标miRNA定期触发两个发夹通过CHA组装成双链体。随后，这些双链体诱导了DNA三向连接的催化组装，并释放了亚甲基蓝标记的DNA链S（S-MB）。此后，通过与捕获探针杂交使用电极捕获S-MB，以产生显着的电流响应。通过改变H0的特定序列，可以响应于不同的miRNA靶标而切换提出的生物传感器。这些发现表明其区分单碱基错配miRNA和分析从细胞中提取的miRNA的能力。因此，本文所述的通用且无酶的信号放大电化学方法可潜在地用于与miRNA相关的各种临床分析和疾病诊断。
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