The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi–bi mechanism,Chemical Communications

当前位置： X-MOL 学术 › Chem. Commun. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi–bi mechanism
Chemical Communications ( IF 4.3 ) Pub Date : 2020/03/09 , DOI: 10.1039/c9cc09887a
Mashael A. Alqasem _{1,

2,

3,

4,

5} , Claire Fergus _{1,

2,

3,

4,

5} , J. Mike Southern _{2,

3,

4,

5,

6} , Stephen J. Connon _{2,

3,

4,

5,

6} , Vincent P. Kelly _{1,

2,

3,

4,

5}

Affiliation

Eukaryotic tRNA-guanine transglycosylase (TGT) – an enzyme recently recognised to be of potential therapeutic importance – catalyses base-exchange of guanine for queuine at the wobble position of tRNAs associated with 4 amino acids via a distinct mechanism to that reported for its eubacterial homologue. The presence of queuine is unequivocally required as a trigger for reaction between the enzyme and tRNA and exhibits cooperativity not seen using guanine as a substrate.

中文翻译：

真核tRNA-鸟嘌呤转糖基酶通过连续的bi-bi机制将奎宁插入tRNA

真核tRNA-鸟嘌呤转糖基酶（TGT）–一种最近被认为具有潜在治疗意义的酶–通过与报道其真细菌同源性不同的机制，催化与4个氨基酸相关的tRNA摆动位置上鸟嘌呤的奎宁碱基交换。。无疑需要奎因的存在作为酶和tRNA之间反应的触发因素，并显示出使用鸟嘌呤作为底物所没有的协同作用。

更新日期：2020-04-03

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11